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题名: Application of a MTT assay for screening nutritional factors in growth media of primary sponge cell culture
作者: Zhang, XY;  Le Pennec, G;  Steffen, R;  Muller, WEG;  Zhang, W
刊名: BIOTECHNOLOGY PROGRESS
发表日期: 2004
DOI: 10.1021/bp0341601
卷: 20, 期:1, 页:151-155
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
英文摘要: Marine sponges (Porifera) are producers of the largest variety of bioactive compounds among benthic marine organisms. In vitro culture of marine sponge cells has been proposed for the sustainable production of these pharmacologically interesting compounds from marine sponges but with limited success. The development of a suitable growth medium is an essential prerequisite for sponge cells grown in vitro. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was adapted to screen for potential nutritional factors in formulating a growth medium for primary cell culture of Suberites domuncula. In 96-well plates, the effects of nutritional factors including glutamine, pyruvate, iron citrate, silicon, RPMI 1640, and Marine Broth 2216 on the viable cell density were examined in primary cell culture of S. domuncula 36 h after inoculation. Ferric iron (Fe3+) and pyruvate were found to significantly improve cell viability in a dose-dependent manner. Silicon and glutamine showed limited improvements at certain concentrations. The supplement of RPMI 1640 and Marine Broth 2216 did not increase cell viability. As a result, several improved media able to maintain higher cell viability in a short-term culture of primary sponge cells could be formulated.
关键词[WOS]: SUBERITES-DOMUNCULA ;  IN-VITRO ;  PRIMMORPHS ;  PROLIFERATION ;  ESTABLISHMENT ;  AGGREGATION ;  CULTIVATION ;  IRON
语种: 英语
WOS记录号: WOS:000188861300022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139567
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Univ Mainz, Abt Angew Molekularbiol, Inst Physiol Chem, D-55099 Mainz, Germany

Recommended Citation:
Zhang, XY,Le Pennec, G,Steffen, R,et al. Application of a MTT assay for screening nutritional factors in growth media of primary sponge cell culture[J]. BIOTECHNOLOGY PROGRESS,2004,20(1):151-155.
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