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Monolithic columns with mixed modes of reversed-phase and anion-exchange stationary phase for capillary electrochromatography
Fu, HJ; Xie, CH; Xiao, H; Dong, J; Hu, JW; Zou, HF
KeywordMonolithic Columns Mixed-mode Separations Stationary Phases Electrochromatography Electrochromatography Anilines Alkylbenzenes Polynuclear Aromatic Hydrocarbons Caffeine Barbitals Benzoic Acids Terephthalic Acid
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2004-07-30
DOI10.1016/j.chroma.2004.04.076
Volume1044Issue:1-2Pages:237-244
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordRIGID POLYMER MONOLITHS ; LIQUID-CHROMATOGRAPHY ; SEPARATION MEDIA ; PEPTIDES ; PROTEINS
AbstractA capillary electrochromatography (CEC) monolithic column with mixed modes of reversed-phase and anion-exchange stationary phases was prepared by in situ polymerization of 2-(methacryloxy)ethyltrimethylammonium methyl sulfate (MEAMS) and ethylene dimethacrylate (EDMA) in a binary porogenic solvent consisting 1-propanol and 1,4-butanediol. The ammonium groups on the surface of the stationary phase generate an electroosmotic flow (EOF) from cathode to anode, and serve as a strong anion-exchange stationary phase at the same time. The EOF of the stationary phase can be determined by the amount of MEAMS monomer in reaction mixtures during the polymerization. The monolithic stationary phases exhibited reversed-phase chromatographic behavior toward neutral solutes. For charged solutes, hydrophobic as well as electrostatic interaction/repulsion with the monoliths was observed. Separations of aromatic compounds and basic compounds on the prepared column were performed under the mode of CEC. Peak tailing of basic compounds was avoided and the efficient separation of aromatic acids was achieved using neutral mobile phase due to the same direction of EOF and electrophorestic mobility of negatively charged solutes. (C) 2004 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000222984300019
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139579
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Fu, HJ,Xie, CH,Xiao, H,et al. Monolithic columns with mixed modes of reversed-phase and anion-exchange stationary phase for capillary electrochromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1044(1-2):237-244.
APA Fu, HJ,Xie, CH,Xiao, H,Dong, J,Hu, JW,&Zou, HF.(2004).Monolithic columns with mixed modes of reversed-phase and anion-exchange stationary phase for capillary electrochromatography.JOURNAL OF CHROMATOGRAPHY A,1044(1-2),237-244.
MLA Fu, HJ,et al."Monolithic columns with mixed modes of reversed-phase and anion-exchange stationary phase for capillary electrochromatography".JOURNAL OF CHROMATOGRAPHY A 1044.1-2(2004):237-244.
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