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题名: Analysis of neutral saccharides by high performance liquid chromatography after pre-column derivatization with 2-(9-carbazole)-ethoxycarbonyl hydrazide
作者: Zhang, L;  Zhang, X;  You, JM;  Duan, JC;  Zhang, WB;  Yan, C;  Zhang, YK
关键词: 2-(9-carbazole)-ethoxycarbonyl hydrazide ;  pre-column derivatization ;  high performance liquid chromatography ;  saccharides
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2004-08-01
卷: 32, 期:8, 页:1002-1005
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: 2-(9-Carbazole)-ethoxycarbonyl hydrazide (CEOC-Hydrazide), a novel pre-column ultraviolet and fluorescence derivatization reagent, has been developed for the analysis of 5 neutral saccharides (lactose, mannose, glucose, rhamnose, sorbose) by high performance liquid chromatography with fluorescence detection and ultraviolet detection. Derivatizing conditions have been investigated. The results indicate that the maximum yields of this derivativation reaction could be obtained within 6.5 h in the presence of 3.5 % (V/V) acetic acid at 65degreesC. The derivatives are detected with fluorescence detection (lambda(ex)/lambda(em) = 293/360 nm) and ultraviolet detection (254 nm). The range of detection limits is from 15 to 60 fmol. It has been proved that this method has excellent sensitivity and the derivatizing reaction of saccharides is easy and operable. The method is suitable for the analysis of saccharides.
关键词[WOS]: DERIVATIVES ;  SUGARS
语种: 英语
WOS记录号: WOS:000224789800005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139600
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu ormal Univ, Dept Chem, Qufu 273165, Peoples R China

Recommended Citation:
Zhang, L,Zhang, X,You, JM,et al. Analysis of neutral saccharides by high performance liquid chromatography after pre-column derivatization with 2-(9-carbazole)-ethoxycarbonyl hydrazide[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2004,32(8):1002-1005.
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