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Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography
Ma, XQ; Wang, LX; Xu, Q; Zhang, F; Xiao, HB; Liang, XM
关键词High Performance Liquid Chromatography(Hplc) Retention Equation Gradient Optimization Ginsenoside
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2004-02-01
25期:2页:238-242
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]ELUTION
英文摘要A multi-segment gradient method for the separation of ginsenosides in reversed phase-high performance liquid chromatography (RP-HPLC) was developed. The retention parameters of 8 ginsenosides (Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2 and Rd) were obtained on an ODS2 C18 column (4.6 mm x 250 mm) from a series of isocratic acetonitrile-water elution. It is found that two-parameter retention equation of ginsenosides can't be used to accurately predict the resolution, but the three-parameter retention equation can. The reason is that the retention factor k' decreases fast while the volume fractions of acetonitrile increase in mobile phase, which make the precision of two-parameter retention equation can't satisfy the predicting need of resolution. Based on the three-parameter retention equation, an optimum elution program consisting of three segments was developed. Under the optimized gradient conditions, the retention time and resolution of ginsenosides are in good agreement with the predicted ones. The analysis time of ginsenosides is shortened and the resolution is improved by the method.
语种英语
WOS记录号WOS:000189187400010
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139601
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Ma, XQ,Wang, LX,Xu, Q,et al. Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2004,25(2):238-242.
APA Ma, XQ,Wang, LX,Xu, Q,Zhang, F,Xiao, HB,&Liang, XM.(2004).Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,25(2),238-242.
MLA Ma, XQ,et al."Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 25.2(2004):238-242.
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