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题名: Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography
作者: Ma, XQ;  Wang, LX;  Xu, Q;  Zhang, F;  Xiao, HB;  Liang, XM
关键词: high performance liquid chromatography(HPLC) ;  retention equation ;  gradient optimization ;  ginsenoside
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2004-02-01
卷: 25, 期:2, 页:238-242
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A multi-segment gradient method for the separation of ginsenosides in reversed phase-high performance liquid chromatography (RP-HPLC) was developed. The retention parameters of 8 ginsenosides (Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2 and Rd) were obtained on an ODS2 C18 column (4.6 mm x 250 mm) from a series of isocratic acetonitrile-water elution. It is found that two-parameter retention equation of ginsenosides can't be used to accurately predict the resolution, but the three-parameter retention equation can. The reason is that the retention factor k' decreases fast while the volume fractions of acetonitrile increase in mobile phase, which make the precision of two-parameter retention equation can't satisfy the predicting need of resolution. Based on the three-parameter retention equation, an optimum elution program consisting of three segments was developed. Under the optimized gradient conditions, the retention time and resolution of ginsenosides are in good agreement with the predicted ones. The analysis time of ginsenosides is shortened and the resolution is improved by the method.
关键词[WOS]: ELUTION
语种: 英语
WOS记录号: WOS:000189187400010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139601
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Ma, XQ,Wang, LX,Xu, Q,et al. Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2004,25(2):238-242.
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