DICP OpenIR
Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography
Ma, XQ; Wang, LX; Xu, Q; Zhang, F; Xiao, HB; Liang, XM
KeywordHigh Performance Liquid Chromatography(Hplc) Retention Equation Gradient Optimization Ginsenoside
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2004-02-01
Volume25Issue:2Pages:238-242
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordELUTION
AbstractA multi-segment gradient method for the separation of ginsenosides in reversed phase-high performance liquid chromatography (RP-HPLC) was developed. The retention parameters of 8 ginsenosides (Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2 and Rd) were obtained on an ODS2 C18 column (4.6 mm x 250 mm) from a series of isocratic acetonitrile-water elution. It is found that two-parameter retention equation of ginsenosides can't be used to accurately predict the resolution, but the three-parameter retention equation can. The reason is that the retention factor k' decreases fast while the volume fractions of acetonitrile increase in mobile phase, which make the precision of two-parameter retention equation can't satisfy the predicting need of resolution. Based on the three-parameter retention equation, an optimum elution program consisting of three segments was developed. Under the optimized gradient conditions, the retention time and resolution of ginsenosides are in good agreement with the predicted ones. The analysis time of ginsenosides is shortened and the resolution is improved by the method.
Language英语
WOS IDWOS:000189187400010
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139601
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Ma, XQ,Wang, LX,Xu, Q,et al. Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2004,25(2):238-242.
APA Ma, XQ,Wang, LX,Xu, Q,Zhang, F,Xiao, HB,&Liang, XM.(2004).Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,25(2),238-242.
MLA Ma, XQ,et al."Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 25.2(2004):238-242.
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