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题名: Chiral speciation and determination of DL-Selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase
作者: Huang, XJ;  Wang, JD;  Wang, QQ;  Huang, BL
刊名: ANALYTICAL SCIENCES
发表日期: 2005-03-01
卷: 21, 期:3, 页:253-257
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A new type of chiral ligand-exchange stationary phase (CLES) was successfully synthesized by treating silica gel with beta-(3,4-epoxycyclohexyl)ethyltrimethoxy silane and opening the epoxy ring by L-isoleucine. The chiral speciation of DL-selenomethionine (DL-SeMet) by high-performance liquid chromatography (HPLC) with UV absorbance on the CLES column was studied. The influences of the contents of copper ion and methanol as well as the pH value in the mobile phase and temperature of the column on the efficiency of resolution Of DL-SeMet were investigated in detail. DL-SeMet could be completely resolved within 40 min under the optimal operating conditions of 0.1 mmol[L CU2+ at 0.05 mol/L KH2PO4 buffer (pH = 5.5) and 35 degrees C temperature of the column. The limits of detection Of D- and L-SeMet were 255 ppb and 286 ppb, respectively. This method was applied to determine the D- and L-enantiomers Of DL-SeMet in real samples, such as selenized yeast powder and garlic.
关键词[WOS]: MASS-SPECTROMETRIC DETECTION ;  ICP-MS ;  CAPILLARY-ELECTROPHORESIS ;  AMINO-ACIDS ;  LIQUID-CHROMATOGRAPHY ;  SELENIUM DEFICIENCY ;  BETA-CYCLODEXTRIN ;  SELENOAMINO ACIDS ;  SEPARATION ;  RESOLUTION
语种: 英语
WOS记录号: WOS:000234120600013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139616
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China
2.Xiamen Univ, MOE Key Lab Analyt Sci, Xiamen 361005, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Huang, XJ,Wang, JD,Wang, QQ,et al. Chiral speciation and determination of DL-Selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase[J]. ANALYTICAL SCIENCES,2005,21(3):253-257.
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