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Chiral speciation and determination of DL-Selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase
Huang, XJ; Wang, JD; Wang, QQ; Huang, BL
Source PublicationANALYTICAL SCIENCES
2005-03-01
Volume21Issue:3Pages:253-257
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRIC DETECTION ; ICP-MS ; CAPILLARY-ELECTROPHORESIS ; AMINO-ACIDS ; LIQUID-CHROMATOGRAPHY ; SELENIUM DEFICIENCY ; BETA-CYCLODEXTRIN ; SELENOAMINO ACIDS ; SEPARATION ; RESOLUTION
AbstractA new type of chiral ligand-exchange stationary phase (CLES) was successfully synthesized by treating silica gel with beta-(3,4-epoxycyclohexyl)ethyltrimethoxy silane and opening the epoxy ring by L-isoleucine. The chiral speciation of DL-selenomethionine (DL-SeMet) by high-performance liquid chromatography (HPLC) with UV absorbance on the CLES column was studied. The influences of the contents of copper ion and methanol as well as the pH value in the mobile phase and temperature of the column on the efficiency of resolution Of DL-SeMet were investigated in detail. DL-SeMet could be completely resolved within 40 min under the optimal operating conditions of 0.1 mmol[L CU2+ at 0.05 mol/L KH2PO4 buffer (pH = 5.5) and 35 degrees C temperature of the column. The limits of detection Of D- and L-SeMet were 255 ppb and 286 ppb, respectively. This method was applied to determine the D- and L-enantiomers Of DL-SeMet in real samples, such as selenized yeast powder and garlic.
Language英语
WOS IDWOS:000234120600013
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139616
Collection中国科学院大连化学物理研究所
Affiliation1.Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China
2.Xiamen Univ, MOE Key Lab Analyt Sci, Xiamen 361005, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Huang, XJ,Wang, JD,Wang, QQ,et al. Chiral speciation and determination of DL-Selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase[J]. ANALYTICAL SCIENCES,2005,21(3):253-257.
APA Huang, XJ,Wang, JD,Wang, QQ,&Huang, BL.(2005).Chiral speciation and determination of DL-Selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase.ANALYTICAL SCIENCES,21(3),253-257.
MLA Huang, XJ,et al."Chiral speciation and determination of DL-Selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase".ANALYTICAL SCIENCES 21.3(2005):253-257.
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