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题名: Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface
作者: Huang, ZX;  Ji, DM;  Xia, AD
关键词: single molecule ;  fluorescence fluctuation ;  reverse intersystem crossing ;  on/off-time ;  dynamic processes
刊名: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
发表日期: 2005-05-05
DOI: 10.1016/j.colsurfa.2004.10.108
卷: 257-58, 页:203-209
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Physical
研究领域[WOS]: Chemistry
英文摘要: Single molecule fluorescence spectroscopy is a powerful tool to reveal the fluorescence fluctuation dynamics of a fluorescence molecule immobilized on a surface. These fluctuations are correlated in time under different excitation intensities with different repetition rate, which may provide the information for us to describe the reasonable excited state processes as well as the surrounding environments. In this report, we showed the first results on the fluorescence fluctuations of a single Cy5 molecule at the air-glass interface excited with different conditions by using far-field confocal laser microscopy in conjunction with multichannel scale (MCS) and time-correlated single photon counting (TCSPC). We found that the on/off-time and count rates showed different behaviors under different excitation modes. It was found that the excitation with the high repetition rate and/or strong intensity may cause high count rate, and increase the possibility of reverse intersystem crossing from T,, to S. state. which may lead to longer on-time and shorter off-time. Furthermore, the photoinduced dynamic processes excited with strong peak power described by a five-level model under continuous wave (CW) and pulsed excitations with high repetition rate, it can be roughly simplified by a three-level scheme under pulse laser at lower repetition rate. (c) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: ROOM-TEMPERATURE ;  CORRELATION SPECTROSCOPY ;  PHOTOPHYSICAL DYNAMICS ;  ELECTRIC DIPOLES ;  QUANTUM JUMPS ;  CYANINE DYES ;  INTERFACE ;  MICROSCOPY ;  EXCITATION
语种: 英语
WOS记录号: WOS:000228321500039
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139644
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China

Recommended Citation:
Huang, ZX,Ji, DM,Xia, AD. Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2005,257-58:203-209.
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