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Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface
Huang, ZX; Ji, DM; Xia, AD
关键词Single Molecule Fluorescence Fluctuation Reverse Intersystem Crossing On/off-time Dynamic Processes
刊名COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
2005-05-05
DOI10.1016/j.colsurfa.2004.10.108
257-58页:203-209
收录类别ISTP ; SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Physical
研究领域[WOS]Chemistry
关键词[WOS]ROOM-TEMPERATURE ; CORRELATION SPECTROSCOPY ; PHOTOPHYSICAL DYNAMICS ; ELECTRIC DIPOLES ; QUANTUM JUMPS ; CYANINE DYES ; INTERFACE ; MICROSCOPY ; EXCITATION
英文摘要Single molecule fluorescence spectroscopy is a powerful tool to reveal the fluorescence fluctuation dynamics of a fluorescence molecule immobilized on a surface. These fluctuations are correlated in time under different excitation intensities with different repetition rate, which may provide the information for us to describe the reasonable excited state processes as well as the surrounding environments. In this report, we showed the first results on the fluorescence fluctuations of a single Cy5 molecule at the air-glass interface excited with different conditions by using far-field confocal laser microscopy in conjunction with multichannel scale (MCS) and time-correlated single photon counting (TCSPC). We found that the on/off-time and count rates showed different behaviors under different excitation modes. It was found that the excitation with the high repetition rate and/or strong intensity may cause high count rate, and increase the possibility of reverse intersystem crossing from T,, to S. state. which may lead to longer on-time and shorter off-time. Furthermore, the photoinduced dynamic processes excited with strong peak power described by a five-level model under continuous wave (CW) and pulsed excitations with high repetition rate, it can be roughly simplified by a three-level scheme under pulse laser at lower repetition rate. (c) 2004 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000228321500039
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被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139644
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China
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Huang, ZX,Ji, DM,Xia, AD. Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2005,257-58:203-209.
APA Huang, ZX,Ji, DM,&Xia, AD.(2005).Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,257-58,203-209.
MLA Huang, ZX,et al."Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 257-58(2005):203-209.
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