中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles
作者: Ye, ZQ;  Tan, MQ;  Wang, GL;  Yuan, JL
关键词: terbium complex ;  fluorescent nanoparticle ;  fluorescence probe ;  time-resolved fluoroimmunoassay
刊名: JOURNAL OF FLUORESCENCE
发表日期: 2005-07-01
DOI: 10.1007/s10895-005-2823-9
卷: 15, 期:4, 页:499-505
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Novel zirconia-based fluorescent terbium nanoparticles have been prepared as a fluorescent nanoprobe for time-resolved fluorescence bioassay. The nanoparticles were prepared in a water-in-oil (W/O) microemulsion consisting of a strongly fluorescent Tb3+ complex, N, N, N-1, N-1-[2,6- bis(3'-aminomethyl-1 - pyrazolyl)- phenylpyridine] tetrakis( acetate)- Tb3+(BPTA-Tb3+), Triton X-100, hexanol, and cyclohexane by controlling co-condensation of Zr(OCH2CH3)(4) and ZrOCl2. The characterizations by transmission electron microscopy and fluorometric methods indicate that the nanoparticles are uniform in size, 33 +/- 4 nm in diameter, and have a fluorescence quantum yield of 8.9% and a long fluorescence lifetime of 2.0 ms. The zirconia-based fluorescent terbium nanoparticles show high stability against basic dissolution in a high pH aqueous buffer compared to the silica-based nanoparticles. A surface modification and bioconjugation method for the fluorescent nanoparticles was developed, and the nanoparticle-conjugated streptavidin (SA) was used for time-resolved floroimmunoassy (TR-FIA) of human prostate specific antigen (PSA). The result shows that the zirconia-based fluorescent terbium nanoparticles are useful as a fluorescent nanoprobe for time-resolved fluorescence bioassay.
关键词[WOS]: TIME-RESOLVED FLUOROIMMUNOASSAY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  MODIFIED POROUS ZIRCONIA ;  BIOCONJUGATED NANOPARTICLES ;  FLUOROMETRIC APPLICATION ;  EUROPIUM NANOPARTICLES ;  AMINO-ACIDS ;  PROTEINS ;  SILICA ;  TEMPERATURE
语种: 英语
WOS记录号: WOS:000231940700006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139646
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Ye, ZQ,Tan, MQ,Wang, GL,et al. Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles[J]. JOURNAL OF FLUORESCENCE,2005,15(4):499-505.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ye, ZQ]'s Articles
 [Tan, MQ]'s Articles
 [Wang, GL]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ye, ZQ]‘s Articles
 [Tan, MQ]‘s Articles
 [Wang, GL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace