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题名: Microarrays of DNA and protein integrated on microfluidic chip
作者: Zhang, ZX;  Shen, Z;  Zhao, H;  Li, B;  Song, SP;  Hu, J;  Lin, BC;  Li, MQ
关键词: microarray ;  microfluidic chip ;  modification ;  polylysine
刊名: ACTA CHIMICA SINICA
发表日期: 2005-09-28
卷: 63, 期:18, 页:1743-1746
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: To combine the advantages of high-throughput microarray and microfluidic chip technology, microarray was fabricated in channels. DNA and protein were arrayed on quartz slide, which was reversibly sealed by poly(dimethylsiloxane) (PDMS) slab containing pattern of channels. The hybridization carried out in channels indicated that target DNA and proteins were bound to their complementary DNA and protein probes specifically. In this method, PDMS conjugated with polylysine was contributed to imparting the wettability and decreasing unspecific adsorption of biomolecules. Our data demonstrated that the integrated chip speeded up the detection compared to microarrays, extended the parallel channels with serial properties and enhanced the throughput of probes and targets.
关键词[WOS]: GENE-EXPRESSION ;  DEVICE ;  ARRAYS ;  PROBES ;  FLOW
语种: 英语
WOS记录号: WOS:000232089800020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139656
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China
4.Shanghai Jiao Tong Univ, Nanobiol Lab, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China

Recommended Citation:
Zhang, ZX,Shen, Z,Zhao, H,et al. Microarrays of DNA and protein integrated on microfluidic chip[J]. ACTA CHIMICA SINICA,2005,63(18):1743-1746.
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