DICP OpenIR
Microarrays of DNA and protein integrated on microfluidic chip
Zhang, ZX; Shen, Z; Zhao, H; Li, B; Song, SP; Hu, J; Lin, BC; Li, MQ
关键词Microarray Microfluidic Chip Modification Polylysine
刊名ACTA CHIMICA SINICA
2005-09-28
63期:18页:1743-1746
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]GENE-EXPRESSION ; DEVICE ; ARRAYS ; PROBES ; FLOW
英文摘要To combine the advantages of high-throughput microarray and microfluidic chip technology, microarray was fabricated in channels. DNA and protein were arrayed on quartz slide, which was reversibly sealed by poly(dimethylsiloxane) (PDMS) slab containing pattern of channels. The hybridization carried out in channels indicated that target DNA and proteins were bound to their complementary DNA and protein probes specifically. In this method, PDMS conjugated with polylysine was contributed to imparting the wettability and decreasing unspecific adsorption of biomolecules. Our data demonstrated that the integrated chip speeded up the detection compared to microarrays, extended the parallel channels with serial properties and enhanced the throughput of probes and targets.
语种英语
WOS记录号WOS:000232089800020
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139656
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China
4.Shanghai Jiao Tong Univ, Nanobiol Lab, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China
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GB/T 7714
Zhang, ZX,Shen, Z,Zhao, H,et al. Microarrays of DNA and protein integrated on microfluidic chip[J]. ACTA CHIMICA SINICA,2005,63(18):1743-1746.
APA Zhang, ZX.,Shen, Z.,Zhao, H.,Li, B.,Song, SP.,...&Li, MQ.(2005).Microarrays of DNA and protein integrated on microfluidic chip.ACTA CHIMICA SINICA,63(18),1743-1746.
MLA Zhang, ZX,et al."Microarrays of DNA and protein integrated on microfluidic chip".ACTA CHIMICA SINICA 63.18(2005):1743-1746.
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