Microfabrication and performance of poly(methyl methacrylate) microfluidic chip with multi-channels
Zhou, XM; Dai, ZP; Luo, Y; Liu, DY; Wang, H; Mao, XL; Lin, BC
KeywordMicrofluldic Plastic Chip Capillary Electrophoresis Chip Microfabrication
Indexed BySCI
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
AbstractThe microchip capillary electrophoresis devices were fabricated by using poly (methyl methacrylate) (PMMA) plastic material via an injection-molding process. The molded devices were enclosed by utilizing a mixed organic solvent to another PMMA film. The channel structure was very well defined and the molded channel surfaces were very smooth. The transmissivity was in the range from 91% to 93% (at the wavelength of 400-1000 nm). In comparison to glass microchannels, the electroosmotic flow(EOF) in native PMMA channels was low. DNA marker separation was demonstrated in these PMMA devices with a high-resolution separation of double-stranded DNA fragments, chip-to-chip and the run-to-run reproducibility was good, and the relative standard deviation (%) values were below 2.2% for run-to-run data and 2.3% for the chip-to-chip comparisons. The PCR amplification products and proteins were analyzed on the PMMA chips. Such devices lead to the production of,low-cost, disposable chips suitable for a variety of separation applications, including DNA sizing, DNA sequencing, protein and medical analysis. The detection limits of Rhodamine 6G dye for the unmodified PMMA chip and the modified PMMA chips were 1.0x10(-10) and 6.67x10(-13) moI/L, respectively.
WOS IDWOS:000226503000015
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhou, XM,Dai, ZP,Luo, Y,et al. Microfabrication and performance of poly(methyl methacrylate) microfluidic chip with multi-channels[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2005,26(1):52-54.
APA Zhou, XM.,Dai, ZP.,Luo, Y.,Liu, DY.,Wang, H.,...&Lin, BC.(2005).Microfabrication and performance of poly(methyl methacrylate) microfluidic chip with multi-channels.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,26(1),52-54.
MLA Zhou, XM,et al."Microfabrication and performance of poly(methyl methacrylate) microfluidic chip with multi-channels".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 26.1(2005):52-54.
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