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Establishment and evaluation of comprehensive two-dimensional liquid chromatography (IEC/RP)
Wang, ZC; Zhang, QH; Zhang, WB; Li, T; Zhao, ZY
关键词Comprehensive Two-dimensional Liquid Chromatography Protein Peak Capacity
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2005-03-10
26期:3页:426-429
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]PROTEINS ; SYSTEM ; INSTRUMENTATION ; INJECTION ; PEPTIDES ; PLATFORM ; PLASMA ; COLUMN
英文摘要A two-dimensional liquid chromatography is established with ion-exchange chromatography (IEC) followed by reversed phase liquid chromatography (RP). The interface is a ten-port two-position switching valve coupled with two parallel RP columns. After splitting the IEC effluent with a ratio of 10 : 1 (volume ratio), the IEC profile and the RP profile were obtained simultaneously. The system is evaluated with 5 standard proteins, which cannot be resolved in either of IEC or RP mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. The total peak capacity of this system is calculated to be 189.
语种英语
WOS记录号WOS:000228021800007
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139678
专题中国科学院大连化学物理研究所
作者单位1.Dalian Elite Analyt Instruments Co Ltd, Dalian 116011, Peoples R China
2.China Univ Geosci, Fac Mat Sci & Chem Engn, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Wang, ZC,Zhang, QH,Zhang, WB,et al. Establishment and evaluation of comprehensive two-dimensional liquid chromatography (IEC/RP)[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2005,26(3):426-429.
APA Wang, ZC,Zhang, QH,Zhang, WB,Li, T,&Zhao, ZY.(2005).Establishment and evaluation of comprehensive two-dimensional liquid chromatography (IEC/RP).CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,26(3),426-429.
MLA Wang, ZC,et al."Establishment and evaluation of comprehensive two-dimensional liquid chromatography (IEC/RP)".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 26.3(2005):426-429.
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