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题名: Establishment and evaluation of comprehensive two-dimensional liquid chromatography (IEC/RP)
作者: Wang, ZC;  Zhang, QH;  Zhang, WB;  Li, T;  Zhao, ZY
关键词: comprehensive two-dimensional liquid chromatography ;  protein ;  peak capacity
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2005-03-10
卷: 26, 期:3, 页:426-429
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A two-dimensional liquid chromatography is established with ion-exchange chromatography (IEC) followed by reversed phase liquid chromatography (RP). The interface is a ten-port two-position switching valve coupled with two parallel RP columns. After splitting the IEC effluent with a ratio of 10 : 1 (volume ratio), the IEC profile and the RP profile were obtained simultaneously. The system is evaluated with 5 standard proteins, which cannot be resolved in either of IEC or RP mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. The total peak capacity of this system is calculated to be 189.
关键词[WOS]: PROTEINS ;  SYSTEM ;  INSTRUMENTATION ;  INJECTION ;  PEPTIDES ;  PLATFORM ;  PLASMA ;  COLUMN
语种: 英语
WOS记录号: WOS:000228021800007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139678
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Elite Analyt Instruments Co Ltd, Dalian 116011, Peoples R China
2.China Univ Geosci, Fac Mat Sci & Chem Engn, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Wang, ZC,Zhang, QH,Zhang, WB,et al. Establishment and evaluation of comprehensive two-dimensional liquid chromatography (IEC/RP)[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2005,26(3):426-429.
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