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Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum
Wang, ZC; Zhang, QG; Zhang, WB; Li, T; Zhao, ZY
关键词Comprehensive Two-dimensional Liquid Chromatography Protein Peak Capacity Goat Serum
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2005-04-01
33期:4页:437-441
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]STATISTICAL-THEORY ; PEPTIDES ; PROTEINS ; INSTRUMENTATION ; OVERLAP ; SYSTEM
英文摘要The system constructed here is a comprehensive two-dimensional liquid chromatography that uses gel filtration chromatography ( GFC) followed by reversed phase liquid chromatography ( RP). Column of Shodex Protein KW-802. 5 (300 mm x 8 mm i. d.) acts as the first dimension which was eluted with 0. 2 mol/L NaH2PO4 ( pH 7. 0) at a flow rate of 0. 15 mL/min. The second dimension uses the column of Hypersil BDS C-18 (35 mm x 4. 6 mm i. d.) which was operated in gradient form. at a flow rate of 3. 0 mL/min. The interface is a 2-position-10-port valve coupled with two parallel columns which analyzed elutes from GFC alternated. The system was evaluated by 5 standard proteins that cannot be-resolved in either GFC or RP mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. The total peak capacity of this system is calculated to be 225. Compared with one-dimensional chromatography, the total peak capacity and the resolution are enhanced. The system was used for separating goat serum to validate and isolate the overlap ingredients.
语种英语
WOS记录号WOS:000228870200001
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139679
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Elite Analyt Instruments Co Ltd, Dalian 116011, Peoples R China
2.China Univ Geosci, Fac Chem Engn & Mat Sci, Wuhan 430074, Peoples R China
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GB/T 7714
Wang, ZC,Zhang, QG,Zhang, WB,et al. Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2005,33(4):437-441.
APA Wang, ZC,Zhang, QG,Zhang, WB,Li, T,&Zhao, ZY.(2005).Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,33(4),437-441.
MLA Wang, ZC,et al."Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 33.4(2005):437-441.
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