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题名: Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum
作者: Wang, ZC;  Zhang, QG;  Zhang, WB;  Li, T;  Zhao, ZY
关键词: comprehensive two-dimensional liquid chromatography ;  protein ;  peak capacity ;  goat serum
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2005-04-01
卷: 33, 期:4, 页:437-441
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The system constructed here is a comprehensive two-dimensional liquid chromatography that uses gel filtration chromatography ( GFC) followed by reversed phase liquid chromatography ( RP). Column of Shodex Protein KW-802. 5 (300 mm x 8 mm i. d.) acts as the first dimension which was eluted with 0. 2 mol/L NaH2PO4 ( pH 7. 0) at a flow rate of 0. 15 mL/min. The second dimension uses the column of Hypersil BDS C-18 (35 mm x 4. 6 mm i. d.) which was operated in gradient form. at a flow rate of 3. 0 mL/min. The interface is a 2-position-10-port valve coupled with two parallel columns which analyzed elutes from GFC alternated. The system was evaluated by 5 standard proteins that cannot be-resolved in either GFC or RP mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. The total peak capacity of this system is calculated to be 225. Compared with one-dimensional chromatography, the total peak capacity and the resolution are enhanced. The system was used for separating goat serum to validate and isolate the overlap ingredients.
关键词[WOS]: STATISTICAL-THEORY ;  PEPTIDES ;  PROTEINS ;  INSTRUMENTATION ;  OVERLAP ;  SYSTEM
语种: 英语
WOS记录号: WOS:000228870200001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139679
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Elite Analyt Instruments Co Ltd, Dalian 116011, Peoples R China
2.China Univ Geosci, Fac Chem Engn & Mat Sci, Wuhan 430074, Peoples R China

Recommended Citation:
Wang, ZC,Zhang, QG,Zhang, WB,et al. Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2005,33(4):437-441.
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