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题名: Separation, identification, and interaction of heparin oligosaccharides with granulocyte-colony stimulating factor using capillary electrophoresis and mass spectrometry
作者: Liang, AY;  Chao, YP;  Liu, XJ;  Du, YG;  Wang, KY;  Qian, SJ;  Lin, BC
关键词: capillary electrophoresis ;  granulocyte-colony stimulating factor ;  heparin oligosaccharides
刊名: ELECTROPHORESIS
发表日期: 2005-09-01
DOI: 10.1002/elps.200500115
卷: 26, 期:18, 页:3460-3467
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A capillary electrophoresis (CE) method was developed for the separation of heparin oligosaccharides compatible to study the interactions between the oligosaccharides and granulocyte-colony stimulating factor (G-CSF). Unfractionated heparin was eliminitively degraded to heparin oligosaccharides by an endolytic heparinase. The degraded smaller oligosaccharides; (M-r < 1000) were baseline-separated by CE under a 50 mm phosphate buffer (pH 9.0) in 10 min. Standard heparin disaccharides and larger oligosaccharides (1000 < M-r < 8000) were all separated under optimized separation conditions. Compared with standard heparin disaccharides, smaller oligosaccharides contained one nonsulfated, two monosulfated, and two disulfated disaccharides, but trisulfated disaccharides were not found. The smaller oligosaccharides were also identified and molecular mass was deduced by electrospray ionization-mass spectrometry (ESI-MS). Furthermore, interactions between G-CSF and the oligosaccharides; were studied by using capillary zone electrophoresis (CZE) under the above separation conditions. It was found that larger oligosaccharides could interact with G-CSF while smaller oligosaccharides were not observed to bind to G-CSF under the experimental conditions. In conclusion, the purified heparinase could selectively degrade heparin into oligosaccharides and the interaction between G-CSF and heparin was correlated with the chain length of heparin.
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ;  GLYCOSAMINOGLYCAN-DERIVED OLIGOSACCHARIDES ;  CHONDROITIN SULFATE OLIGOSACCHARIDES ;  INDUCED FLUORESCENCE DETECTION ;  COMPOSITIONAL ANALYSIS ;  ASSOCIATION CONSTANTS ;  GEL-ELECTROPHORESIS ;  PROTEIN INTERACTION ;  BINDING SEQUENCE ;  DISACCHARIDES
语种: 英语
WOS记录号: WOS:000232351800007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139708
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Acad Life Sci, Shanghai, Peoples R China

Recommended Citation:
Liang, AY,Chao, YP,Liu, XJ,et al. Separation, identification, and interaction of heparin oligosaccharides with granulocyte-colony stimulating factor using capillary electrophoresis and mass spectrometry[J]. ELECTROPHORESIS,2005,26(18):3460-3467.
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