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题名: Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry
作者: Yang, J;  Xu, GW;  Zheng, WF;  Kong, HW;  Wang, C;  Zhao, XJ;  Pang, T
关键词: metabonomics ;  peak alignment ;  liver ;  cancer ;  pattern recognition
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2005-08-19
DOI: 10.1016/j.chroma.2005.10.100
卷: 1084, 期:1-2, 页:214-221
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Metabonomics, the study of metabolites and their roles in various disease states, is a novel methodology arising from the post-genomics era. This methodology has been applied in many fields. Current metabonomic practice has relied on mass spectrometry (MS), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) to analyze metabolites. In this study, a strategy was developed for applying high-performance liquid chromatography (HPLC) and LC-MS-MS to metabonomics research. One of the key problems to be solved in this strategy is to match the peaks between the chromatograms. A peak alignment algorithm has been developed to match the chromatograms before the pattern recognition. As an application example, the strategy described above was applied to metabonomics research on liver diseases, and the false-positive result of live cancer diagnosis from the hepatocirrhosis and hepatitis diseases was effectively reduced to 7.40%. Based on the pattern recognition, several potential biomarkers were found and further identified by the following LC-MS-MS experiments. The structures of eight potential biomarkers were given for distinguishing the liver cancer from the hepatocirrhosis and hepatitis diseases. (c) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: NEURAL-NETWORK CLASSIFICATION ;  CAPILLARY-ELECTROPHORESIS ;  FUNCTIONAL GENOMICS ;  MODIFIED NUCLEOSIDES ;  METABOLOME ANALYSIS ;  GAS-CHROMATOGRAPHY ;  ALIGNMENT ;  PROFILES ;  EXTRACTS ;  URINARY
语种: 英语
WOS记录号: WOS:000230862600031
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139722
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Yang, J,Xu, GW,Zheng, WF,et al. Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1084(1-2):214-221.
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