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Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry
Yang, J; Xu, GW; Zheng, WF; Kong, HW; Wang, C; Zhao, XJ; Pang, T
KeywordMetabonomics Peak Alignment Liver Cancer Pattern Recognition
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2005-08-19
DOI10.1016/j.chroma.2005.10.100
Volume1084Issue:1-2Pages:214-221
Indexed ByISTP ; SCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordNEURAL-NETWORK CLASSIFICATION ; CAPILLARY-ELECTROPHORESIS ; FUNCTIONAL GENOMICS ; MODIFIED NUCLEOSIDES ; METABOLOME ANALYSIS ; GAS-CHROMATOGRAPHY ; ALIGNMENT ; PROFILES ; EXTRACTS ; URINARY
AbstractMetabonomics, the study of metabolites and their roles in various disease states, is a novel methodology arising from the post-genomics era. This methodology has been applied in many fields. Current metabonomic practice has relied on mass spectrometry (MS), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) to analyze metabolites. In this study, a strategy was developed for applying high-performance liquid chromatography (HPLC) and LC-MS-MS to metabonomics research. One of the key problems to be solved in this strategy is to match the peaks between the chromatograms. A peak alignment algorithm has been developed to match the chromatograms before the pattern recognition. As an application example, the strategy described above was applied to metabonomics research on liver diseases, and the false-positive result of live cancer diagnosis from the hepatocirrhosis and hepatitis diseases was effectively reduced to 7.40%. Based on the pattern recognition, several potential biomarkers were found and further identified by the following LC-MS-MS experiments. The structures of eight potential biomarkers were given for distinguishing the liver cancer from the hepatocirrhosis and hepatitis diseases. (c) 2004 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000230862600031
Citation statistics
Cited Times:74[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139722
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Yang, J,Xu, GW,Zheng, WF,et al. Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1084(1-2):214-221.
APA Yang, J.,Xu, GW.,Zheng, WF.,Kong, HW.,Wang, C.,...&Pang, T.(2005).Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1084(1-2),214-221.
MLA Yang, J,et al."Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1084.1-2(2005):214-221.
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