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题名: Simultaneous measurements of the flow velocities in a microchannel by wide/evanescent field illuminations with particle/single molecules
作者: Gai, HW;  Li, Y;  Silber-Li, Z;  Ma, YF;  Lin, BC
刊名: LAB ON A CHIP
发表日期: 2005
DOI: 10.1039/b416476h
卷: 5, 期:4, 页:443-449
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: A laser-induced fluorescence imaging method was developed to simultaneously measure flow velocities in the middle and near wall of a channel with particles or single molecules, by selectively switching from the wide field excitation mode to the evanescent wave excitation mode. Fluorescent microbeads with a diameter of 175 nm were used to calibrate the system, and the collisions of microbeads with channel walls were directly observed. The 175 nm microbeads velocities in the main flow and at 275 nm from the bottom of the channel were measured. The measured velocities of particles or single molecules in two positions in a microchannel were consistent with the calculated value based on Poiseuille flow theory when the diameter of a microbead was considered. The errors caused by Brownian diffusion in our measurement were negligible compared to the flow velocity. Single lDNA molecules were then used as a flowing tracer to measure the velocities. The velocity can be obtained at a distance of 309.0 +/- 82.6 nm away from bottom surface of the channel. The technique may be potentially useful for studying molecular transportation both in the center and at the bottom of the channel, and interactions between molecules and microchannel surfaces. It is especially important that the technique can be permitted to measure both velocities in the same experiment to eliminate possible experimental inconsistencies.
关键词[WOS]: SINGLE-DNA-MOLECULES ;  FLUORESCENCE SPECTROSCOPY ;  CAPILLARY-ELECTROPHORESIS ;  IMAGING TECHNIQUES ;  ROOM-TEMPERATURE ;  RADIAL MIGRATION ;  MICROFLUIDICS ;  DYNAMICS ;  CHIP
语种: 英语
WOS记录号: WOS:000227893100011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139743
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100080, Peoples R China
3.Univ Missouri, Rolla, MO 65409 USA

Recommended Citation:
Gai, HW,Li, Y,Silber-Li, Z,et al. Simultaneous measurements of the flow velocities in a microchannel by wide/evanescent field illuminations with particle/single molecules[J]. LAB ON A CHIP,2005,5(4):443-449.
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