DICP OpenIR
Simultaneous measurements of the flow velocities in a microchannel by wide/evanescent field illuminations with particle/single molecules
Gai, HW; Li, Y; Silber-Li, Z; Ma, YF; Lin, BC
Source PublicationLAB ON A CHIP
2005
DOI10.1039/b416476h
Volume5Issue:4Pages:443-449
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordSINGLE-DNA-MOLECULES ; FLUORESCENCE SPECTROSCOPY ; CAPILLARY-ELECTROPHORESIS ; IMAGING TECHNIQUES ; ROOM-TEMPERATURE ; RADIAL MIGRATION ; MICROFLUIDICS ; DYNAMICS ; CHIP
AbstractA laser-induced fluorescence imaging method was developed to simultaneously measure flow velocities in the middle and near wall of a channel with particles or single molecules, by selectively switching from the wide field excitation mode to the evanescent wave excitation mode. Fluorescent microbeads with a diameter of 175 nm were used to calibrate the system, and the collisions of microbeads with channel walls were directly observed. The 175 nm microbeads velocities in the main flow and at 275 nm from the bottom of the channel were measured. The measured velocities of particles or single molecules in two positions in a microchannel were consistent with the calculated value based on Poiseuille flow theory when the diameter of a microbead was considered. The errors caused by Brownian diffusion in our measurement were negligible compared to the flow velocity. Single lDNA molecules were then used as a flowing tracer to measure the velocities. The velocity can be obtained at a distance of 309.0 +/- 82.6 nm away from bottom surface of the channel. The technique may be potentially useful for studying molecular transportation both in the center and at the bottom of the channel, and interactions between molecules and microchannel surfaces. It is especially important that the technique can be permitted to measure both velocities in the same experiment to eliminate possible experimental inconsistencies.
Language英语
WOS IDWOS:000227893100011
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139743
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100080, Peoples R China
3.Univ Missouri, Rolla, MO 65409 USA
Recommended Citation
GB/T 7714
Gai, HW,Li, Y,Silber-Li, Z,et al. Simultaneous measurements of the flow velocities in a microchannel by wide/evanescent field illuminations with particle/single molecules[J]. LAB ON A CHIP,2005,5(4):443-449.
APA Gai, HW,Li, Y,Silber-Li, Z,Ma, YF,&Lin, BC.(2005).Simultaneous measurements of the flow velocities in a microchannel by wide/evanescent field illuminations with particle/single molecules.LAB ON A CHIP,5(4),443-449.
MLA Gai, HW,et al."Simultaneous measurements of the flow velocities in a microchannel by wide/evanescent field illuminations with particle/single molecules".LAB ON A CHIP 5.4(2005):443-449.
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