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题名: Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay
作者: Yuan, JL;  Wang, GL
关键词: lanthanide complex ;  fluorescence label ;  time-resolved fluorescence bioassay
刊名: JOURNAL OF FLUORESCENCE
发表日期: 2005-07-01
DOI: 10.1007/s10895-005-2829-3
卷: 15, 期:4, 页:559-568
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Different from organic fluorescence dyes, fluorescent lanthanide complexes have the fluorescence properties of long fluorescence lifetime, large Stokes shift and sharp emission profile, which makes them favorable be used as the fluorescent labeling reagents for microsecond time-resolved fluorescence bioassay. Lanthanide complex-based fluorescence labels have been successfully used for highly sensitive time-resolved fluorescence immunoassay, DNA hybridization assay, cell activity assay, and bioimaging microscopy assay. Since the technique allows easy distinction of the specific fluorescence signal of the long-lived label from short-lived background noises associated with biological samples, scattering lights (Tyndall, Rayleigh and Raman scatterings) and the optical components (cuvettes, filters and lenses), the sensitivity of fluorescence bioassay has been remarkably improved. This paper summarized the recent developments of lanthanide complex-based fluorescence labels and their applications in time-resolved fluorescence bioassays mainly based on the authors' researches and relative publications.
关键词[WOS]: DIKETONATE-EUROPIUM CHELATE ;  PROSTATE-SPECIFIC ANTIGEN ;  HIGHLY SENSITIVE DETECTION ;  DNA HYBRIDIZATION ASSAY ;  STATE ENERGY-LEVEL ;  FLUOROMETRIC APPLICATIONS ;  LUMINESCENCE PROPERTIES ;  ENVIRONMENTAL-SAMPLES ;  QUANTITATIVE MEASUREMENT ;  BIOANALYTICAL ASSAYS
语种: 英语
WOS记录号: WOS:000231940700012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139765
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Yuan, JL,Wang, GL. Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay[J]. JOURNAL OF FLUORESCENCE,2005,15(4):559-568.
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