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题名: Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge
作者: Zhang, CZ;  Zhang, YF;  Chen, JP;  Liang, XM
关键词: purification ;  characterization ;  baicalin-p-D-glucuronidase ;  Scutellaria viscidula Bge
刊名: PROCESS BIOCHEMISTRY
发表日期: 2005-04-01
DOI: 10.1016/j.procbio.2004.07.003
卷: 40, 期:5, 页:1911-1915
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering
英文摘要: The baicalin-beta-D-glucuronidase that hydrolyzes the beta-D-glucuronide bond of baicalin to form baicalein was isolated from fresh roots of Scutellaria viscidula Bge and the enzyme purified and characterized. The enzyme was purified to one band on SDS-polyacrylamide gel electrophoresis and its molecular weight was about 58.4 kDa. The optimum temperature of the baicalin-p-D-glucuronidase was 50 degrees C, and the optimum pH was 5.0. The baicalin-beta-D-glucuronidase was stable at less than 70 degrees C and pH 4.0-7.0. Ca2+ and Zn2+ ions have no significant effect on enzyme activity, Mg2+ ion has a weakly positive effect and Cu2+ ion has a weakly negative effect on enzyme activity, while Fe3+ ion inhibits enzyme activity strongly. (c) 2004 Elsevier Ltd. All rights reserved.
关键词[WOS]: WOGONIN ;  GEORGI ;  RADIX ;  FLAVONE
语种: 英语
WOS记录号: WOS:000227569000055
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139768
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Inst Light Ind, Coll Bio & Food Technol, Dalian 116034, Peoples R China

Recommended Citation:
Zhang, CZ,Zhang, YF,Chen, JP,et al. Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge[J]. PROCESS BIOCHEMISTRY,2005,40(5):1911-1915.
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