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Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge
Zhang, CZ; Zhang, YF; Chen, JP; Liang, XM
KeywordPurification Characterization Baicalin-p-d-glucuronidase Scutellaria Viscidula Bge
Source PublicationPROCESS BIOCHEMISTRY
2005-04-01
DOI10.1016/j.procbio.2004.07.003
Volume40Issue:5Pages:1911-1915
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS KeywordWOGONIN ; GEORGI ; RADIX ; FLAVONE
AbstractThe baicalin-beta-D-glucuronidase that hydrolyzes the beta-D-glucuronide bond of baicalin to form baicalein was isolated from fresh roots of Scutellaria viscidula Bge and the enzyme purified and characterized. The enzyme was purified to one band on SDS-polyacrylamide gel electrophoresis and its molecular weight was about 58.4 kDa. The optimum temperature of the baicalin-p-D-glucuronidase was 50 degrees C, and the optimum pH was 5.0. The baicalin-beta-D-glucuronidase was stable at less than 70 degrees C and pH 4.0-7.0. Ca2+ and Zn2+ ions have no significant effect on enzyme activity, Mg2+ ion has a weakly positive effect and Cu2+ ion has a weakly negative effect on enzyme activity, while Fe3+ ion inhibits enzyme activity strongly. (c) 2004 Elsevier Ltd. All rights reserved.
Language英语
WOS IDWOS:000227569000055
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139768
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Inst Light Ind, Coll Bio & Food Technol, Dalian 116034, Peoples R China
Recommended Citation
GB/T 7714
Zhang, CZ,Zhang, YF,Chen, JP,et al. Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge[J]. PROCESS BIOCHEMISTRY,2005,40(5):1911-1915.
APA Zhang, CZ,Zhang, YF,Chen, JP,&Liang, XM.(2005).Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge.PROCESS BIOCHEMISTRY,40(5),1911-1915.
MLA Zhang, CZ,et al."Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge".PROCESS BIOCHEMISTRY 40.5(2005):1911-1915.
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