DICP OpenIR
Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge
Zhang, CZ; Zhang, YF; Chen, JP; Liang, XM
关键词Purification Characterization Baicalin-p-d-glucuronidase Scutellaria Viscidula Bge
刊名PROCESS BIOCHEMISTRY
2005-04-01
DOI10.1016/j.procbio.2004.07.003
40期:5页:1911-1915
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]WOGONIN ; GEORGI ; RADIX ; FLAVONE
英文摘要The baicalin-beta-D-glucuronidase that hydrolyzes the beta-D-glucuronide bond of baicalin to form baicalein was isolated from fresh roots of Scutellaria viscidula Bge and the enzyme purified and characterized. The enzyme was purified to one band on SDS-polyacrylamide gel electrophoresis and its molecular weight was about 58.4 kDa. The optimum temperature of the baicalin-p-D-glucuronidase was 50 degrees C, and the optimum pH was 5.0. The baicalin-beta-D-glucuronidase was stable at less than 70 degrees C and pH 4.0-7.0. Ca2+ and Zn2+ ions have no significant effect on enzyme activity, Mg2+ ion has a weakly positive effect and Cu2+ ion has a weakly negative effect on enzyme activity, while Fe3+ ion inhibits enzyme activity strongly. (c) 2004 Elsevier Ltd. All rights reserved.
语种英语
WOS记录号WOS:000227569000055
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139768
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Inst Light Ind, Coll Bio & Food Technol, Dalian 116034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, CZ,Zhang, YF,Chen, JP,et al. Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge[J]. PROCESS BIOCHEMISTRY,2005,40(5):1911-1915.
APA Zhang, CZ,Zhang, YF,Chen, JP,&Liang, XM.(2005).Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge.PROCESS BIOCHEMISTRY,40(5),1911-1915.
MLA Zhang, CZ,et al."Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge".PROCESS BIOCHEMISTRY 40.5(2005):1911-1915.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, CZ]的文章
[Zhang, YF]的文章
[Chen, JP]的文章
百度学术
百度学术中相似的文章
[Zhang, CZ]的文章
[Zhang, YF]的文章
[Chen, JP]的文章
必应学术
必应学术中相似的文章
[Zhang, CZ]的文章
[Zhang, YF]的文章
[Chen, JP]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。