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题名: A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system
作者: Yang, BC;  Tan, F;  Guan, YF
关键词: light-emitting diode ;  fluorescence ;  collinear
刊名: SPECTROSCOPY AND SPECTRAL ANALYSIS
发表日期: 2005-12-01
卷: 25, 期:12, 页:1995-1997
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Spectroscopy
研究领域[WOS]: Spectroscopy
英文摘要: A compact light-emitting diode-induced fluorescence detector based on collinear scheme is described. The detector was assembled by all-solid-state optical-electronic components and easily coupled with micro-scale flow analysis system by on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids were used as test analytes to evaluate the detector performance by capillary electrophoresis and flow injection analysis techniques. The concentration limit of detection was 10(-8) mol (circle) L-1 for FTC-labeled phenylalanine at a signal-to-noise ratio (S/N) of 3 (on-column, 0. 05 mm i.d.). The system exhibited a good linear response in the range of 1 X 10(-7) -2 X 10(-5) mol (circle) L-1 (r(2) = 0.999). The relative standard deviation (RSD) of 6 repetitive injections on peak heights is less than 3 %.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  WAVE-GUIDE
语种: 英语
WOS记录号: WOS:000234388500023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139779
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Yang, BC,Tan, F,Guan, YF. A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2005,25(12):1995-1997.
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