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题名: Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region
作者: Zhang, XC;  Liang, YB;  Fan, JF;  Zhang, W;  Pu, HY;  Liang, B;  Chen, HX;  Song, LC
关键词: Perkinsus ;  PCR ;  ITS ;  Manila clam
刊名: ACTA OCEANOLOGICA SINICA
发表日期: 2005
卷: 24, 期:5, 页:139-144
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Oceanography
研究领域[WOS]: Oceanography
英文摘要: Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, China. The internal transcribed spacer(ITS) in rDNA was PCR-amplified, cloned, sequenced, and compared with that of five Perkinsus species in GenBank. The fragment amplified from DNA of parasite of either Shicheng Island or Ningbo contained 649 bp, including partial ssrRNA(51 bp) and ITS(+5.8 S) (598 bp) regions. The ITS(+5.8S) sequences of Perkinsus-like parasite of both Shicheng Island and Ningbo were all 99% identical to those of Perkinsus atlanticus,and were not more than 95% identical to those of other four Perkinsus species including P. marinus, P. andrewsi, P. qugwadi and P. medierraneus. The ITS (+5.8S) sequence of Perkinsus-like parasite of Shicheng Island was 99% identical to that of Ningbo. These facts about nucleotide sequences suggested that the Perkinsus-like parasite in Manila clam, Ruditapes philippinarum collected from either the Huanghai Sea coast or the East China Sea coast was P. atlanticus, and might reflect P. adanticus strains of distinct geographic distribution.
关键词[WOS]: RIBOSOMAL-RNA LOCUS ;  DIAGNOSTIC ASSAY ;  APICOMPLEXA ;  ATLANTICUS ;  MARINUS
语种: 英语
WOS记录号: WOS:000232432700016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139782
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.State Ocean Adm, Inst Marine Environm Protect, Dalian 116023, Peoples R China
3.Dalian Fisheries Univ, Life Sci & Technol Inst, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, XC,Liang, YB,Fan, JF,et al. Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region[J]. ACTA OCEANOLOGICA SINICA,2005,24(5):139-144.
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