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Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region
Zhang, XC; Liang, YB; Fan, JF; Zhang, W; Pu, HY; Liang, B; Chen, HX; Song, LC
关键词Perkinsus Pcr Its Manila Clam
刊名ACTA OCEANOLOGICA SINICA
2005
24期:5页:139-144
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Oceanography
研究领域[WOS]Oceanography
关键词[WOS]RIBOSOMAL-RNA LOCUS ; DIAGNOSTIC ASSAY ; APICOMPLEXA ; ATLANTICUS ; MARINUS
英文摘要Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, China. The internal transcribed spacer(ITS) in rDNA was PCR-amplified, cloned, sequenced, and compared with that of five Perkinsus species in GenBank. The fragment amplified from DNA of parasite of either Shicheng Island or Ningbo contained 649 bp, including partial ssrRNA(51 bp) and ITS(+5.8 S) (598 bp) regions. The ITS(+5.8S) sequences of Perkinsus-like parasite of both Shicheng Island and Ningbo were all 99% identical to those of Perkinsus atlanticus,and were not more than 95% identical to those of other four Perkinsus species including P. marinus, P. andrewsi, P. qugwadi and P. medierraneus. The ITS (+5.8S) sequence of Perkinsus-like parasite of Shicheng Island was 99% identical to that of Ningbo. These facts about nucleotide sequences suggested that the Perkinsus-like parasite in Manila clam, Ruditapes philippinarum collected from either the Huanghai Sea coast or the East China Sea coast was P. atlanticus, and might reflect P. adanticus strains of distinct geographic distribution.
语种英语
WOS记录号WOS:000232432700016
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139782
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.State Ocean Adm, Inst Marine Environm Protect, Dalian 116023, Peoples R China
3.Dalian Fisheries Univ, Life Sci & Technol Inst, Dalian 116023, Peoples R China
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Zhang, XC,Liang, YB,Fan, JF,et al. Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region[J]. ACTA OCEANOLOGICA SINICA,2005,24(5):139-144.
APA Zhang, XC.,Liang, YB.,Fan, JF.,Zhang, W.,Pu, HY.,...&Song, LC.(2005).Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region.ACTA OCEANOLOGICA SINICA,24(5),139-144.
MLA Zhang, XC,et al."Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region".ACTA OCEANOLOGICA SINICA 24.5(2005):139-144.
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