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题名: On-column refolding and purification of recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) expressed as inclusion body in Escherichia coli
作者: Tan, HD;  Dan, GP;  Gong, HY;  Cao, LJ
关键词: human interleukin-1 receptor antagonist ;  inclusion body ;  on-column refolding
刊名: BIOTECHNOLOGY LETTERS
发表日期: 2005-08-01
DOI: 10.1007/s10529-005-8655-5
卷: 27, 期:16, 页:1177-1182
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a 44% recovery, without an intermediate dialysis step. Refolded rHuIL-1ra had full biological activity with the MTT assay. An intramolecular disulfide linkage in the oxidized recombinant protein was suggested by data from HPLC and non-reducing SDS-PAGE.
关键词[WOS]: INTERLEUKIN-1 RECEPTOR ANTAGONIST ;  ANAKINRA ;  PROTEIN
语种: 英语
WOS记录号: WOS:000231856500005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139785
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Yushui Biopharmaceut Co Ltd, Chongqing 200400, Peoples R China

Recommended Citation:
Tan, HD,Dan, GP,Gong, HY,et al. On-column refolding and purification of recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) expressed as inclusion body in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2005,27(16):1177-1182.
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