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题名: Up-regulation of fibronectin in oesophageal squamous cell carcinoma is associated with activation of the Erk pathway
作者: Zhang, J;  Zhi, HY;  Zhou, CN;  Ding, F;  Luo, AP;  Zhang, X;  Sun, YT;  Wang, XQ;  Wu, M;  Liu, ZH
关键词: oesophageal squamous cell carcinoma ;  fibronectin ;  pErk-1/2 ;  pRaf-1 ;  MMP-2 ;  invasion
刊名: JOURNAL OF PATHOLOGY
发表日期: 2005-12-01
DOI: 10.1002/path.1846
卷: 207, 期:4, 页:402-409
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Oncology ;  Pathology
研究领域[WOS]: Oncology ;  Pathology
英文摘要: Fibronectin (FN) was found to be up-regulated in human oesophageal squamous cell carcinoma (ESCC) by cDNA microarray analysis in our laboratory. In order to elucidate the chronology of FN expression at various stages of oesophageal carcinogenesis, RTPCR, immunohistochemistry and Western blot analysis were carried out on ESCC tissue samples with different pathological characteristics. FN was mainly localized in the interstitial tissues, and its up-regulation in ESCC was significantly associated with the depth of invasion by carcinoma (R = 0.803, p < 0.01). To investigate its relationship with the Erk pathway further, pRaf-1 and pErk-1/2 expression were also analysed in ESCC. Activation of Erk1/2 and Raf was identified in 63.3% and 60.3% of the tumour specimens, respectively, whereas normal mucosal epithelial tissues were negative. Moreover, a close association was observed between pErk-1/2 expression and the differentiation grade (R = -0.421, p = 0.002): pErk-1/2 signal was greater in poorly differentiated tissues than in well and moderately differentiated tissues. Co-expression of FN and pErk-1/2 was found at the invasive front of tumour nests by double immunofluorescence staining and there was a statistical correlation between the expression of FN and pErk-1/2 (p < 0.05). In the cell line EC9706, plasma FN was able to phosphorylate Raf and further activate Erk, but it did not alter MMP-2 protein expression or activity, indicating that MMP-2 may not be the downstream target gene of the Erk pathway. All the above data suggest that the up-regulation of FN contributes to the later stage of oesophageal carcinogenesis, and that activation of the Erk pathway may be involved in the roles of FN in ESCC. Copyright (c) 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
关键词[WOS]: BREAST-CANCER ;  MAP-KINASES ;  EXPRESSION ;  PROTEIN ;  PROGRESSION ;  TUMOR ;  FRAGMENTS ;  INTEGRIN ;  ADHESION ;  INVASION
语种: 英语
WOS记录号: WOS:000233760000004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139792
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Canc, Natl Lab Mol Oncol, Beijing 100021, Peoples R China
2.Peking Union Med Coll, Beijing 100021, Peoples R China
3.Hosp, Chinese Acad Sci, Dept Pathol, Beijing 100021, Peoples R China
4.Chinese Acad Sci, Canc Inst & Hosp, Natl Lab Mol Oncol, Beijing 100021, Peoples R China
5.Hebei Normal Univ, Coll Life Sci, Shijiazhuang, Peoples R China

Recommended Citation:
Zhang, J,Zhi, HY,Zhou, CN,et al. Up-regulation of fibronectin in oesophageal squamous cell carcinoma is associated with activation of the Erk pathway[J]. JOURNAL OF PATHOLOGY,2005,207(4):402-409.
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