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题名: Construction of a new method for mobilizing the zones of CIEF and its application
作者: Zhu, GJ;  Yang, C;  Liu, HC;  Zhang, LH;  Zhang, WB;  Zhang, YK
关键词: capillary isoelectric focusing(CIEF) ;  electroosmotic flow(EOF) ;  protein ;  polypeptide
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2005-04-10
卷: 26, 期:4, 页:654-656
收录类别: SCI
文章类型: Letter
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A simple method for mobilizing the focused zones of capillary isoelectric focusing (CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated capillary by an etched porous joint. The etched section allows electrical conductivity but blocks the hydrodynamic flow. The focused zones of mixed proteins was mobilized by this method successfully. Compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  MASS-SPECTROMETRY ;  ETCHED JOINT ;  INTERFACE ;  PERFORMANCE ;  PROTEINS
语种: 英语
WOS记录号: WOS:000228675700016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139818
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhu, GJ,Yang, C,Liu, HC,et al. Construction of a new method for mobilizing the zones of CIEF and its application[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2005,26(4):654-656.
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