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题名: Preparation of silica-bonded bovine serum albumin chiral monolithic column for enantiomeric separation by capillary electrochromatography
作者: Yan, LJ;  Zhang, QH;  Zhang, WB;  Feng, YQ;  Zhang, LH;  Li, T;  Zhang, YK
关键词: capillary electrochromatography(CEC) ;  monolithic column ;  sol-gel ;  chiral stationary phases (CSps) ;  bovine serum albumin (BSA)
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2005-05-10
卷: 26, 期:5, 页:835-837
收录类别: SCI
文章类型: Letter
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A silica-bonded bovine serum albumin (BSA) chiral monolithic stationary phase for capillary electrochromatography(CEC) was introduced. An inorganic-organic hybrid monolithic column was firstly prepared by sol-gel chemistry with homogeneously distributed aminopropyl groups throughout the silica matrix. Then the chiral stationary phase was synthesized by the in situ covalent immobilization of BSA on the monolithic column activated with glutaraldehyde. The effects of pH value and concentration of phosphate buffer on the separation of D,L-tryptophan were investigated. The separation factor of D,L-tryptophan reached 3.37 on CEC mode.
关键词[WOS]: SOL-GEL PROCESS
语种: 英语
WOS记录号: WOS:000229296100012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139822
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
3.Dalian Elite Analyt Instruments Co Ltd, Dalian 116011, Peoples R China

Recommended Citation:
Yan, LJ,Zhang, QH,Zhang, WB,et al. Preparation of silica-bonded bovine serum albumin chiral monolithic column for enantiomeric separation by capillary electrochromatography[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2005,26(5):835-837.
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