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题名: Adipose tissue model using three-dimensional cultivation of preadipocytes seeded onto fibrous polymer scaffolds
作者: Kang, XH;  Xie, YB;  Kniss, DA
刊名: TISSUE ENGINEERING
发表日期: 2005-03-01
卷: 11, 期:3-4, 页:458-468
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell & Tissue Engineering
研究领域[WOS]: Cell Biology
英文摘要: A better understanding of the mechanism of adipose tissue differentiation is of paramount importance in the development of therapeutic strategies for the treatment and prevention of obesity and type 2 diabetes mellitus. Optimal results using tissue culture models can be expected only when the in vitro adipocyte resembles adipose tissue in vivo as closely as possible. In this study, we used tissue-engineering principles to develop a three-dimensional (3-D) culture system to mimic the geometry of adipose tissue in vivo. Mouse preadipocyte 3T3-L1 cells were seeded onto nonbiodegradable fibrous polyethylene terephthalate scaffolds and differentiated with a hormone cocktail consisting of insulin, dexamethasone, isobutylmethylxanthine, and fetal calf serum. Cell morphology, growth, differentiation, and function were studied by immunocytochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, enzyme-linked immunosorbent assay, and oil red O staining. Cells grown on 3-D fibrous scaffolds were differentiated in situ by hormone induction with high efficiency (approximately 90%) as shown by scanning electron microscopy. Immunocytochemistry, immunoblot analysis, and RT-PCR revealed that the 3- D constructs expressed adipocyte-specific genes, including peroxisome proliferator-activated receptor γ, leptin, adipsin, aP2, adiponectin, GLUT4, and resistin. Adipocytes matured on 3-D constructs secreted leptin at levels even greater than that of fully differentiated adipocytes in 2-D conventional cell cultures. Finally, adipocyte-specific phenotypic function was demonstrated by accumulation of neutral lipids in larger fat droplets. In conclusion, preadipocytes grown on 3-D matrices acquire morphology and biological features of mature adipocytes. This new culture model should have significant utility for in vitro studies of adipocyte cell biology and development.
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ;  ADIPOCYTE DIFFERENTIATION ;  IN-VITRO ;  CLINICAL-APPLICATIONS ;  EXTRACELLULAR-MATRIX ;  GENE-EXPRESSION ;  CELL ;  ADIPOGENESIS ;  CULTURE ;  LEPTIN
语种: 英语
WOS记录号: WOS:000228999400013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139833
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Ohio State Univ, Coll Med & Publ Hlth, Dept Obstet & Gynecol, Lab Perinatal Res, Columbus, OH 43210 USA
2.Ohio State Univ, Coll Engn, Ctr Biomed Engn, Columbus, OH 43210 USA
3.Ohio State Univ, Coll Med & Publ Hlth, Ctr Biomed Engn, Columbus, OH 43210 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian, Peoples R China

Recommended Citation:
Kang, XH,Xie, YB,Kniss, DA. Adipose tissue model using three-dimensional cultivation of preadipocytes seeded onto fibrous polymer scaffolds[J]. TISSUE ENGINEERING,2005,11(3-4):458-468.
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