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题名: Determination of urinary 8-hydroxy-2 '-deoxyguanosine by two approaches - capillary electrophoresis and GUMS: An assay for in vivo oxidative DNA damage in cancer patients
作者: Mei, SR;  Yao, QH;  Wu, CY;  Xu, GW
关键词: 8-hydroxy-2 '-deoxyguanosine ;  cancer ;  capillary electrophoresis ;  GC/MS
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2005-11-15
DOI: 10.1016/j.jchromb.2005.04.001
卷: 827, 期:1, 页:83-87
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: 8-Hydroxy-2'-deoxyguanosine (8OHdG) has been considered as an excellent marker of oxidative DNA damage associated with age-related diseases such as cancer. In this paper, two sensitive methods-capillary electrophoresis with electrochemical detection (CE-ECD) and gas chromatography/mass spectrometry (GC/MS) were developed for urinary 8OHdG analysis. The R.S.D. of the spiked recovery of the two 41111111 methods for determining urinary 8OHdG was 4.03% and 8.25%, respectively, and the results from the two methods have a good consistency ( r= 0.999, P < 0.01). The developed CE-ECD method was applied to investigate the urinary 8OHAG levels in different cancer patients and follow up the response of therapy. It was found that the excretion levels of urinary 8OHdG in cancer patients were significantly higher than those in healthy persons (35.26 +/- 27.96 nM versus 13.51 +/- 5.08 nM, P < 0.05), and cancer patients receiving surgical therapy and chemotherapy showed a significant decrease in urinary 8OHdG. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ELECTROCHEMICAL DETECTION ;  GAS-CHROMATOGRAPHY ;  BIOMARKER EVIDENCE ;  MASS-SPECTROMETRY ;  8-HYDROXYDEOXYGUANOSINE ;  CARCINOGENESIS ;  8-HYDROXYGUANINE ;  EXCRETION ;  STRESS
语种: 英语
WOS记录号: WOS:000233327100012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139842
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China
2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

Recommended Citation:
Mei, SR,Yao, QH,Wu, CY,et al. Determination of urinary 8-hydroxy-2 '-deoxyguanosine by two approaches - capillary electrophoresis and GUMS: An assay for in vivo oxidative DNA damage in cancer patients[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,827(1):83-87.
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