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Determination of urinary 8-hydroxy-2 '-deoxyguanosine by two approaches - capillary electrophoresis and GUMS: An assay for in vivo oxidative DNA damage in cancer patients
Mei, SR; Yao, QH; Wu, CY; Xu, GW
关键词8-hydroxy-2 '-deoxyguanosine Cancer Capillary Electrophoresis Gc/ms
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2005-11-15
DOI10.1016/j.jchromb.2005.04.001
827期:1页:83-87
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROCHEMICAL DETECTION ; GAS-CHROMATOGRAPHY ; BIOMARKER EVIDENCE ; MASS-SPECTROMETRY ; 8-HYDROXYDEOXYGUANOSINE ; CARCINOGENESIS ; 8-HYDROXYGUANINE ; EXCRETION ; STRESS
英文摘要8-Hydroxy-2'-deoxyguanosine (8OHdG) has been considered as an excellent marker of oxidative DNA damage associated with age-related diseases such as cancer. In this paper, two sensitive methods-capillary electrophoresis with electrochemical detection (CE-ECD) and gas chromatography/mass spectrometry (GC/MS) were developed for urinary 8OHdG analysis. The R.S.D. of the spiked recovery of the two 41111111 methods for determining urinary 8OHdG was 4.03% and 8.25%, respectively, and the results from the two methods have a good consistency ( r= 0.999, P < 0.01). The developed CE-ECD method was applied to investigate the urinary 8OHAG levels in different cancer patients and follow up the response of therapy. It was found that the excretion levels of urinary 8OHdG in cancer patients were significantly higher than those in healthy persons (35.26 +/- 27.96 nM versus 13.51 +/- 5.08 nM, P < 0.05), and cancer patients receiving surgical therapy and chemotherapy showed a significant decrease in urinary 8OHdG. (c) 2005 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000233327100012
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被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139842
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China
2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
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Mei, SR,Yao, QH,Wu, CY,et al. Determination of urinary 8-hydroxy-2 '-deoxyguanosine by two approaches - capillary electrophoresis and GUMS: An assay for in vivo oxidative DNA damage in cancer patients[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,827(1):83-87.
APA Mei, SR,Yao, QH,Wu, CY,&Xu, GW.(2005).Determination of urinary 8-hydroxy-2 '-deoxyguanosine by two approaches - capillary electrophoresis and GUMS: An assay for in vivo oxidative DNA damage in cancer patients.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,827(1),83-87.
MLA Mei, SR,et al."Determination of urinary 8-hydroxy-2 '-deoxyguanosine by two approaches - capillary electrophoresis and GUMS: An assay for in vivo oxidative DNA damage in cancer patients".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 827.1(2005):83-87.
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