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Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis
Qin, JH; Liu, ZY; Wu, DP; Zhu, N; Zhou, XM; Fung, YS; Lin, BC
KeywordAngiotensinogen Gene Genotyping Microchip Electrophoresis Miniaturization Polymorphism Analysis
Source PublicationELECTROPHORESIS
2005
DOI10.1002/elps.200406158
Volume26Issue:1Pages:219-224
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordESSENTIAL-HYPERTENSION ; M235T ; ASSOCIATION ; VARIANT ; DNA ; LINKAGE ; DISEASE ; SITE
AbstractAngiotensinogen (AGT) gene has been regarded as one of the candidate genes for essential hypertension. In our study, the role of AGT gene as a putatively predisposing gene for hypertension was evaluated by genotyping a A (-6) G polymorphism in the core promoter region in 123 patients with essential hypertension and 103 healthy controls. A microchip electrophoresis method coupled with polymorphism chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay was used for genotyping the A (-6) G single-nucleotide polymorphism. The separation and detection of the digested PCR amplicons were completed just in 280 s or less. The genotype frequency fulfilled the criteria of the Hardy-Weinbery equilibrium (X-2 = 3.067, P > 0.05). The results showed a higher frequency of the -6 A allele (0.70) in the normotensive subjects, which is higher than those reported in Germany (0.47) and Czech (0.40) populations, but similar to that found in Japanese populations (0.73). The frequencies of genotype AA, AG, and GG were 0.46, 0.49, and 0.05 in hypertensive subjects, and 0.44, 0.53, and 0.03 in control subjects. There is no significant difference in the distributions of the genotype and allele between the two groups (X-2 = 0.88, P > 0.05; X-2 = 0.024, P > 0.05). These findings differ from some of the results obtained in other ethnic groups, indicating the potential importance of ethnic origin in the assessment of genetic risk identifiers for a complex disease.
Language英语
WOS IDWOS:000226467400028
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Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139844
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 1, Dept Pediat, Dalian, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Cardiol, Dalian, Peoples R China
4.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Qin, JH,Liu, ZY,Wu, DP,et al. Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis[J]. ELECTROPHORESIS,2005,26(1):219-224.
APA Qin, JH.,Liu, ZY.,Wu, DP.,Zhu, N.,Zhou, XM.,...&Lin, BC.(2005).Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis.ELECTROPHORESIS,26(1),219-224.
MLA Qin, JH,et al."Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis".ELECTROPHORESIS 26.1(2005):219-224.
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