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题名: Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis
作者: Qin, JH;  Liu, ZY;  Wu, DP;  Zhu, N;  Zhou, XM;  Fung, YS;  Lin, BC
关键词: angiotensinogen gene ;  genotyping ;  microchip electrophoresis ;  miniaturization ;  polymorphism analysis
刊名: ELECTROPHORESIS
发表日期: 2005
DOI: 10.1002/elps.200406158
卷: 26, 期:1, 页:219-224
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Angiotensinogen (AGT) gene has been regarded as one of the candidate genes for essential hypertension. In our study, the role of AGT gene as a putatively predisposing gene for hypertension was evaluated by genotyping a A (-6) G polymorphism in the core promoter region in 123 patients with essential hypertension and 103 healthy controls. A microchip electrophoresis method coupled with polymorphism chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay was used for genotyping the A (-6) G single-nucleotide polymorphism. The separation and detection of the digested PCR amplicons were completed just in 280 s or less. The genotype frequency fulfilled the criteria of the Hardy-Weinbery equilibrium (X-2 = 3.067, P > 0.05). The results showed a higher frequency of the -6 A allele (0.70) in the normotensive subjects, which is higher than those reported in Germany (0.47) and Czech (0.40) populations, but similar to that found in Japanese populations (0.73). The frequencies of genotype AA, AG, and GG were 0.46, 0.49, and 0.05 in hypertensive subjects, and 0.44, 0.53, and 0.03 in control subjects. There is no significant difference in the distributions of the genotype and allele between the two groups (X-2 = 0.88, P > 0.05; X-2 = 0.024, P > 0.05). These findings differ from some of the results obtained in other ethnic groups, indicating the potential importance of ethnic origin in the assessment of genetic risk identifiers for a complex disease.
关键词[WOS]: ESSENTIAL-HYPERTENSION ;  M235T ;  ASSOCIATION ;  VARIANT ;  DNA ;  LINKAGE ;  DISEASE ;  SITE
语种: 英语
WOS记录号: WOS:000226467400028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139844
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 1, Dept Pediat, Dalian, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Cardiol, Dalian, Peoples R China
4.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Qin, JH,Liu, ZY,Wu, DP,et al. Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis[J]. ELECTROPHORESIS,2005,26(1):219-224.
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