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题名: Kinetic study of the mass transfer of bovine serum albumin on cibacron blue cellulose membranes by using the multi-plate and transport models
作者: Hao, WQ;  Wang, JD
关键词: column liquid chromatography ;  membrane affinity chromatography ;  frontal analysis ;  multi-plate model ;  transport modelling
刊名: CHROMATOGRAPHIA
发表日期: 2005-07-01
DOI: 10.1365/s10337-005-0584-x
卷: 62, 期:1-2, 页:55-62
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The mass transfer kinetics of bovine serum albumin on Cibacron blue F3GA cellulose affinity membranes has been investigated. It was found that the multi-plate (MP) and transport models successfully described experimental breakthrough curves obtained by single-step frontal analysis. The correlation between the two models was used to estimate the rate coefficients of mass transfer from experimental data. The flow rate was found to have little effect on the performance of affinity membrane separations. The improvement of the performance by increasing the thickness of the membranes was limited. The transport model was simplified by approximating the very sharp Langmuir isotherm determined by a rectangular isotherm and an analytical solution obtained although this was found to be unsuited to describe the experimental data.
关键词[WOS]: TRANSFER RATE COEFFICIENT ;  AFFINITY-CHROMATOGRAPHY ;  CONCENTRATION-DEPENDENCE ;  LIQUID-CHROMATOGRAPHY ;  MATHEMATICAL-ANALYSIS ;  PROTEIN SEPARATIONS ;  FRONTAL ANALYSIS ;  PURIFICATION ;  ADSORPTION ;  PERFORMANCE
语种: 英语
WOS记录号: WOS:000230748400009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139845
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Hao, WQ,Wang, JD. Kinetic study of the mass transfer of bovine serum albumin on cibacron blue cellulose membranes by using the multi-plate and transport models[J]. CHROMATOGRAPHIA,2005,62(1-2):55-62.
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