DICP OpenIR
Kinetic study of the mass transfer of bovine serum albumin on cibacron blue cellulose membranes by using the multi-plate and transport models
Hao, WQ; Wang, JD
KeywordColumn Liquid Chromatography Membrane Affinity Chromatography Frontal Analysis Multi-plate Model Transport Modelling
Source PublicationCHROMATOGRAPHIA
2005-07-01
DOI10.1365/s10337-005-0584-x
Volume62Issue:1-2Pages:55-62
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordTRANSFER RATE COEFFICIENT ; AFFINITY-CHROMATOGRAPHY ; CONCENTRATION-DEPENDENCE ; LIQUID-CHROMATOGRAPHY ; MATHEMATICAL-ANALYSIS ; PROTEIN SEPARATIONS ; FRONTAL ANALYSIS ; PURIFICATION ; ADSORPTION ; PERFORMANCE
AbstractThe mass transfer kinetics of bovine serum albumin on Cibacron blue F3GA cellulose affinity membranes has been investigated. It was found that the multi-plate (MP) and transport models successfully described experimental breakthrough curves obtained by single-step frontal analysis. The correlation between the two models was used to estimate the rate coefficients of mass transfer from experimental data. The flow rate was found to have little effect on the performance of affinity membrane separations. The improvement of the performance by increasing the thickness of the membranes was limited. The transport model was simplified by approximating the very sharp Langmuir isotherm determined by a rectangular isotherm and an analytical solution obtained although this was found to be unsuited to describe the experimental data.
Language英语
WOS IDWOS:000230748400009
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139845
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Hao, WQ,Wang, JD. Kinetic study of the mass transfer of bovine serum albumin on cibacron blue cellulose membranes by using the multi-plate and transport models[J]. CHROMATOGRAPHIA,2005,62(1-2):55-62.
APA Hao, WQ,&Wang, JD.(2005).Kinetic study of the mass transfer of bovine serum albumin on cibacron blue cellulose membranes by using the multi-plate and transport models.CHROMATOGRAPHIA,62(1-2),55-62.
MLA Hao, WQ,et al."Kinetic study of the mass transfer of bovine serum albumin on cibacron blue cellulose membranes by using the multi-plate and transport models".CHROMATOGRAPHIA 62.1-2(2005):55-62.
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