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题名: K-ras gene mutation detection by single-strand conformation polymorphism with capillary electrophoresis
作者: Shi, XZ;  Li, JH;  Zhao, CX;  Lu, S;  Yang, Q;  Xu, GW
关键词: short-chain linear polyacrylamide ;  capillary electrophoresis ;  single-strand conformation polymorphism ;  K-ras gene
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2005-02-01
卷: 33, 期:2, 页:177-180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Mutation of K-ras gene plays an important role in colorectal cancer tumorigenesis. Short-chain linear polyacrylamide (SLPA) was synthesized using isopropanol as a chain transfer agent. The viscosity coefficient and molecular mass of SLPA were 0.70 x 10(-3) m(3)/kg and 6.5 x 10(4) respectively. A single-strand conformation polymorphism with laser-induced fluorescence capillary electrophoresis method for detection of K-ras gene mutation was developed under the conditions of 6% SLPA, running temperature at 27 degrees C running voltage at 9 kV. By this method, tumor tissues from 36 colorectal cancer patient were screened, and twelve of them were found to be K-ras gene mutation. The results revealed that this method is rapid and high sensitivity and become a promising tool to screen a large number of samples in clinical diagnosis of colorectal cancer.
关键词[WOS]: LASER-INDUCED FLUORESCENCE
语种: 英语
WOS记录号: WOS:000227651300008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139847
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China

Recommended Citation:
Shi, XZ,Li, JH,Zhao, CX,et al. K-ras gene mutation detection by single-strand conformation polymorphism with capillary electrophoresis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2005,33(2):177-180.
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