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题名: Rapid microvolume PCR of DNA confirmed by microchip electrophoresis
作者: Hataoka, Y;  Zhang, LH;  Yukimasa, T;  Baba, Y
刊名: ANALYTICAL SCIENCES
发表日期: 2005
卷: 21, 期:1, 页:53-56
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: PCR is an indispensable technique used in DNA analysis. However. with the traditional methods. the time spent on amplification and the subsequent analysis of PCR products is generally long. Therefore. it is essential to improve these two steps so that the whole procedure can be made faster. In the present work, with lambda-DNA as the control template., the amplification of 300-bp fragment could be completed within 37 s with capillary reaction chambers of LightCycler. and the following analysis of PCR products could be completed within 120 s with microchip electrophoresis as the detector. Since the high detection sensitivity of microchip electrophoresis, PCR products with template concentration as low as 5 fg/muL could be detected only after 435 s of amplification. In addition. based on additional optimized conditions simulated by CoventorWare, PCR microchips with distinct structure of the reaction chambers have been designed and successfully applied to the amplification of 300-bp fragment. By comparison, those chambers with ellipse and racket shapes were found to offer very high amplification efficiency. All of these results demonstrate the promise of integrating PCR and electrophoresis on microchip for developing easy-carrying instruments for the fast in situ detection of DNA.
关键词[WOS]: POLYMERASE-CHAIN-REACTION ;  BETA-GLOBIN ;  AMPLIFICATION ;  DEVICE ;  SENSITIVITY ;  DIAGNOSIS
语种: 英语
WOS记录号: WOS:000226362300011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139860
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Tokushima, 21st Century COE, CREST,Grad Sch Pharmaceut Sci, JST,Dept Mol & Pharmaceut Biotechnol, Tokushima 7708505, Japan
2.Matsushita Elect Ind Co Ltd, Adv Technol Res Labs, Kyoto 6190237, Japan
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
4.Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Chikusa Ku, Nagoya, Aichi 4648603, Japan
5.Natl Inst Adv Ind Sci & Technol, Single Mol Bioanal Lab, Takamatsu, Kagawa 7610395, Japan

Recommended Citation:
Hataoka, Y,Zhang, LH,Yukimasa, T,et al. Rapid microvolume PCR of DNA confirmed by microchip electrophoresis[J]. ANALYTICAL SCIENCES,2005,21(1):53-56.
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