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题名: Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum
作者: Dong, J;  Xie, CH;  Tian, RJ;  Wu, RN;  Hu, JW;  Zou, HF
关键词: basic drugs ;  electrochromatography ;  electrophoretic mobility ;  human serum ;  neutral monolith
刊名: ELECTROPHORESIS
发表日期: 2005-09-01
DOI: 10.1002/elps.200500273
卷: 26, 期:18, 页:3452-3459
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Capillary electrochromatography (CEC) using a neutral hydrophobic polymer-based monolithic column has been developed for classification of analytes based on their acidity and charges of group. The monolithic columns were prepared by in situ copolymerization of lauryl methacrylate and ethylene dimethacrylate without any charged monomers in the reaction mixture. The ionic analytes will only be driven by their electrophoretic mobilities and were separated on the basis of their differences in electrophoretic mobility and in hydrophobic interaction with the stationary phase. Only negatively or positively charged analyte (acid or basic) migrated toward detection window in a single run by applying negative or positive voltage. The CEC system was also applied for the analysis of basic drugs in human serum by internal standard method using acidic running buffer. The sample of human serum spiked with basic drugs was directly injected after a simple sample pretreatment. The calibration curves with regression coefficients (0.9995-0.9997) in the range of 0.318-80 mu g/mL were obtained with the limits of detection being below 0.15 mu g/mL. The intra- and inter-day precisions, determined as relative standard deviations, were less than 4.21%.
关键词[WOS]: POROUS POLYMER MONOLITHS ;  LIQUID-CHROMATOGRAPHY ;  SEPARATION MEDIA ;  STATIONARY PHASES ;  ELECTROPHORESIS ;  PERFORMANCE ;  PRESSURE ;  DRIVEN
语种: 英语
WOS记录号: WOS:000232351800006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139874
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Dong, J,Xie, CH,Tian, RJ,et al. Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum[J]. ELECTROPHORESIS,2005,26(18):3452-3459.
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