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题名: Optimization of Saccharomyces cerevisiae culture in alginate-chitosan-alginate microcapsule
作者: Qi, WT;  Yu, WT;  Xie, YB;  Ma, XJ
关键词: microencapsulation ;  ACA microcapsule ;  Saccharomyces cerevisiae ;  cell growth
刊名: BIOCHEMICAL ENGINEERING JOURNAL
发表日期: 2005-09-01
DOI: 10.1016/j.bej.2005.04.019
卷: 25, 期:2, 页:151-157
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Engineering
英文摘要: Microencapsulation is one of the promising methods for microorganism immobilization. The parameters of microencapsulation have impact on the growth and performance of microencapsulated microorganism. In this paper, the effects of microcapsules core state (solid or liquid), initial cell density (1.5 x 10(7), 3 x 10(6) and 3 x 10(5) cells/ml of microcapsules), microcapsule diameter (200, 500, 600 and 700 mu m) and membrane formation times (0, 5, 15 and 30 min) on cell growth, including proliferating capacity, metabolic activity and product secretion of Saccharomyces cerevisiae, cultured in alginate-chitosan-alginate (ACA) microcapsule, were investigated. The results showed that there was no significant difference in cell growth of microencapsulated cells, whether the core of the microcapsules was solid or liquefied. Increase in inoculate cell density shortened the lag phase time of cell growth, while cell density obtained 25 times of the initial density of 3 x 10(6) cells/ml of microcapsule, which was the highest. Increase in microcapsules size had no significant impact on cell proliferation, metabolism and product secretion, but the leakage of the bacteria was the least when the microcapsule size was 600 mu m in diameter. The increase in membrane formation time reduced the leakage of cells. It was demonstrated that the optimized parameters for microencapsulated S. cerevisiae culture were initial cell density of 3 x 10(6) cells/ml of microcapsule, microcapsule size of 600 mu m in diameter, and membrane formation time of 15 min. This study provides useful information for intestinal delivery of therapeutic agents from genetically modified food-grade microorganisms, using microencapsulation. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: ARTIFICIAL CELLS ;  MICROENCAPSULATION ;  ENCAPSULATION ;  MEMBRANE ;  SURVIVAL ;  DELIVERY ;  BEADS
语种: 英语
WOS记录号: WOS:000231493900006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139878
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China

Recommended Citation:
Qi, WT,Yu, WT,Xie, YB,et al. Optimization of Saccharomyces cerevisiae culture in alginate-chitosan-alginate microcapsule[J]. BIOCHEMICAL ENGINEERING JOURNAL,2005,25(2):151-157.
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