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题名: On-line isotachophoretic preconcentration and gel electrophoretic separation of sodium dodecyl sulfate-proteins on a microchip
作者: Huang, HQ;  Xu, F;  Dai, ZP;  Lin, BC
关键词: gel electrophoresis ;  isotachophoresis ;  miniaturization ;  on-line preconcentration ;  sodium dodecyl sulfate-proteins
刊名: ELECTROPHORESIS
发表日期: 2005-06-01
DOI: 10.1002/elps.200410393
卷: 26, 期:11, 页:2254-2260
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A microchip for integrated isotachophoretic (ITP) preconcentration with gel electrophoretic (GE) separation to decrease the detectable concentration of sodium dodecyl sulfate (SDS)-proteins was developed. Each channel of the chip was designed with a long sample injection channel to increase the sample loading and allow stacking the sample into a narrow zone using discontinuous ITP buffers. The preconcentrated sample was separated in GE mode in sieving polymer solutions. All the analysis steps including injection, preconcentration, and separation of the ITP-GE process were performed continuously, controlled by a high-voltage power source with sequential voltage switching between the analysis steps. Without deteriorating the peak resolution, four SDS-protein analyses with integrated ITP-GE system resulted in a decreased detectable concentration of similar to 40-fold compared to the GE mode only. A good calibration curve for molecular weights of SIDS-proteins indicated that the integrated ITP-GE system can be used for qualitative analysis of unknown protein samples.
关键词[WOS]: TOTAL ANALYSIS SYSTEMS ;  COLUMN-COUPLING CHIP ;  CAPILLARY ZONE ELECTROPHORESIS ;  MICROFLUIDIC DEVICES ;  CONDUCTIVITY DETECTION ;  PLANAR CHIP ;  CHANNELS ;  COMPLEXES ;  SAMPLES ;  DNA
语种: 英语
WOS记录号: WOS:000229901500023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139915
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China

Recommended Citation:
Huang, HQ,Xu, F,Dai, ZP,et al. On-line isotachophoretic preconcentration and gel electrophoretic separation of sodium dodecyl sulfate-proteins on a microchip[J]. ELECTROPHORESIS,2005,26(11):2254-2260.
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