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题名: Multilayer poly(vinyl alcohol)-adsorbed coating on poly(dimethylsiloxane) microfluidic chips for biopolymer separation
作者: Wu, DP;  Luo, Y;  Zhou, XM;  Dai, ZP;  Lin, BC
关键词: microfluidic chip ;  miniaturization ;  poly(dimethylsiloxane) ;  poly(vinyl alcohol)
刊名: ELECTROPHORESIS
发表日期: 2005
DOI: 10.1002/elps.200406157
卷: 26, 期:1, 页:211-218
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A poly(dimethylsiloxane) (PDMS) microfluidic chip surface was modified by multilayer-adsorbed and heat-immobilized poly(vinyl alcohol) (PVA) after oxygen plasma treatment. The reflection absorption infrared spectrum (RAIRS) showed that 88% hydrolyzed PVA adsorbed more strongly than 100% hydrolyzed one on the oxygen plasma-pretreated PDMS surface, and they all had little adsorption on original PDMS surface. Repeating the coating procedure three times was found to produce the most robust and effective coating. PVA coating converted the original PDMS surface from a hydrophobic one into a hydrophilic surface, and suppressed electroosmotic flow (EOF) in the range of pH 3-11. More than 1 000 000 plates/m and baseline resolution were obtained for separation of fluorescently labeled basic proteins (lysozyme, ribonuclease B). Fluorescently labeled acidic proteins (bovine serum albumin, P-lactoglobulin) and fragments of dsDNA phiX174 RF/HaeIII were also separated satisfactorily in the three-layer 88% PVA-coated PDMS microchip. Good separation of basic proteins was obtained for about 70 consecutive runs.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  MICROCHIP ELECTROPHORESIS ;  SURFACE MODIFICATION ;  ELECTROOSMOTIC FLOW ;  DEVICES ;  ALCOHOL) ;  SYSTEMS ;  PROTEINS ;  FABRICATION ;  CHANNELS
语种: 英语
WOS记录号: WOS:000226467400027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139920
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Wu, DP,Luo, Y,Zhou, XM,et al. Multilayer poly(vinyl alcohol)-adsorbed coating on poly(dimethylsiloxane) microfluidic chips for biopolymer separation[J]. ELECTROPHORESIS,2005,26(1):211-218.
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