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题名: Characterization of small-molecule-biomicromolecule interactions: From simple to complex
作者: Tian, RJ;  Xu, SY;  Lei, XY;  Jin, WH;  Ye, ML;  Zou, HF
关键词: analytical methodologies ;  library screening ;  multiple recognition systems ;  simple ligand-receptor binding ;  small-molecule-biomacromolecule interaction
刊名: TRAC-TRENDS IN ANALYTICAL CHEMISTRY
发表日期: 2005-10-01
DOI: 10.1016/j.trac.2005.03.018
卷: 24, 期:9, 页:810-825
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The interactions between small molecules and biomacromolecules constitute one of the most important parts of interconnected biological networks. To understand the recognition mechanisms of these interactions is of vital importance for characterizing the expression and the function of related biomacromolecules and further discovering drug candidates for the pharmaceutical industry and biomarkers for clinical diagnosis. Here, we summarize the general methodology used in these studies, mainly including chromatographic, electromigration and mass spectrometric methods. We present the latest applications of these methods for elucidating simple binding of plasma protein, enzyme, receptor and DNA/RNA with small molecules. We also present recognition studies based on multi-component systems (e.g., phage display, oligonucleotides, chemiproteomics and traditional Chinese medicines), which should be more challenging in both analytical tools and methodology and the function of biological systems. (c) 2005 Elsevier Ltd. All rights reserved.
关键词[WOS]: HUMAN SERUM-ALBUMIN ;  IONIZATION MASS-SPECTROMETRY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  AFFINITY CAPILLARY-ELECTROPHORESIS ;  CDNA-PHAGE DISPLAY ;  IMMOBILIZED-ENZYME REACTOR ;  SURFACE-PLASMON RESONANCE ;  DISSOCIATION-CONSTANTS ;  COMPETITIVE-BINDING ;  STATIONARY PHASES
语种: 英语
WOS记录号: WOS:000232970400010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139931
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Tian, RJ,Xu, SY,Lei, XY,et al. Characterization of small-molecule-biomicromolecule interactions: From simple to complex[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2005,24(9):810-825.
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