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题名: Study on interaction between antibiotics and Escherichia coli DH5 alpha by microcalorimetric method
作者: Yang, L. N.;  Xu, F.;  Sun, L. X.;  Tan, Z. C.;  Tan, H. D.;  Zhao, Z. B.;  Liang, J. G.
关键词: E.coli DH5 alpha ;  inhibition ;  microcalorimetry ;  Piperacillin Sodium ;  Sulbactam Sodium
刊名: JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
发表日期: 2006-09-01
DOI: 10.1007/s10973-006-7507-4
卷: 85, 期:3, 页:807-810
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical ;  Chemistry, Physical
研究领域[WOS]: Chemistry
英文摘要: A microcalorimetric technique based on the bacterial heat output was applied to evaluate the influence of antibiotics PIP (Piperacillin Sodium) and composite preparation of PIP and SBT (Sulbactam Sodium) on the growth of E. coli DH5 alpha. The power-time curves of the growth metabolism of E. coli DH5 alpha were studied using a TAM Air Isothermal Microcalorimeter at 37 degrees C. By analyzing the power-time curves, the parameters such as growth rate constants (k), inhibitory ratio (I), the maximum heat power (P-m) and the time of the maximum heat power (t (m)) were obtained. The results show that different concentrations of antibiotics affect the growth metabolism of E. coli DH5 alpha. The PIP in the concentration range of 0-0.05 mu g mL(-1) has a stimulatory effect on the E. coli DH5 alpha growth, while the PIP of higher concentrations (0.05 -0.25 mu g mL(-1)) can inhibit its growth. It seems that the composite preparation composed of PIP and SBT cannot improve the inhibitory effect on E. coli DH5 alpha as compared with the PIP.
关键词[WOS]: SULBACTAM SODIUM ;  PROMOTER ;  GROWTH ;  ENTHALPY
语种: 英语
WOS记录号: WOS:000240554500048
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139947
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
4.Hunan Inst Drug Detect, Changsha 410001, Peoples R China

Recommended Citation:
Yang, L. N.,Xu, F.,Sun, L. X.,et al. Study on interaction between antibiotics and Escherichia coli DH5 alpha by microcalorimetric method[J]. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY,2006,85(3):807-810.
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