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题名: A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity
作者: Wang, JH;  Jing, K;  Wei, L;  Molchanova, V;  Chikalovets, I;  Belogortseva, N;  Luk'yanov, P;  Zheng, YT
关键词: HIV-1 ;  antiviral activity ;  entry inhibitor ;  CVL ;  beta-galactose-specific lectin ;  natural products ;  marine invertebrates ;  Chaetopterus variopedatus
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
发表日期: 2006
DOI: 10.1016/j.cbpc.2005.10.019
卷: 142, 期:1-2, 页:111-117
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism ;  Toxicology ;  Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism ;  Toxicology ;  Zoology
英文摘要: A 30 kDa beta-galactose-specific lectin named CVL was isolated from the polychaete marine worm Chaetopterus variopedatus (Annelida) and its anti-HIV-1 activity in vitro was determined. Results showed that CVL inhibited cytopathic effect induced by HIV-1 and the production of viral p24 antigen. The EC50 values were 0.0043 and 0.057 mu M, respectively. Time-of-addition analysis of anti-HIV-1 activity indicated its action was at the early stage of virus replication. CVL could blocked the cell-to-cell fusion process of HIV infected and uninfected cells with an EC50 of 0.073 mu M. The inhibition of HIV-1 entry into host cells was demonstrated by using fluorescence-based real-time quantify PCR. At CVL concentration of 0.33 mu M and 0.07 mu M, 86% and 21% virus attachment were blocked, respectively. The anti-HIV-1 action of CVL might relate to blockade of HIV-1 entry into cells. (c) 2005 Elsevier Inc. All rights reserved.
关键词[WOS]: HUMAN-IMMUNODEFICIENCY-VIRUS ;  ENVELOPE GLYCOPROTEIN GP120 ;  BINDING LECTIN ;  CYANOVIRIN-N ;  ANTIVIRAL ACTIVITIES ;  CUCUMARIA-ECHINATA ;  PLANT-LECTINS ;  T-CELLS ;  PURIFICATION ;  MANNOSE
语种: 英语
WOS记录号: WOS:000235916600013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139950
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Zool, Lab Mol Immunopharmacol, Kunming 650223, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Russian Acad Sci, Pacific Inst Bioorgan Chem, Far E Branch, Vladivostok 690022, Russia
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Wang, JH,Jing, K,Wei, L,et al. A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2006,142(1-2):111-117.
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