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题名: Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail
作者: Luan, Hongwei;  Liu, Xin;  Qi, Xiaohui;  Hu, Ying;  Hao, Dacheng;  Cui, Yu;  Yang, Ling
关键词: beta-D-glucosidase ;  thermo- and pH-stability ;  ginsenoside Rb-1 ;  ginsenoside Rd ;  purification ;  China white jade snail
刊名: PROCESS BIOCHEMISTRY
发表日期: 2006-09-01
DOI: 10.1016/j.procbio.2006.04.011
卷: 41, 期:9, 页:1974-1980
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering
英文摘要: A highly stable beta-glucosidase from the viscera of China white jade snail was purified to apparent homogeneity. This purified glucosidase consisted of two identical subunits with a native molecular mass of approximately 230 kDa. The maximal activity to p-nitrophenyl-beta-(D)-glucopyranoside (pNPG) occurred at 70 degrees C and pH 5.6. Its most notable characteristic was stable over a wide pH range (3-11 at 30 degrees C for 24 h) and a relatively high temperature (60 degrees C for 24 h). The K-m for pNPG was 0.338 mM and the V-max was 0.25 mmol nitrophenol/min/mg glucosidase at pH 5.6 and 50 degrees C. Compared to its activity against pNPG (100%), the beta-glucosidase exhibited low levels of activity against other aryl-glycosides (less than 1%). Additionally, the enzyme hydrolyzed the 20-C, beta-(1 -> 6)-glucoside of ginsenoside Rb-1 to produce ginsenoside Rd, but did not hydrolyze the other beta-(D)-glucosidic bonds of Rb-1. The properties of the enzyme could make it become a useful tool in biotransformation of glucosides. (c) 2006 Elsevier Ltd. All rights reserved.
关键词[WOS]: THERMOPHILIC FUNGUS ;  RD ;  SAPONINS ;  PROTEIN ;  METABOLISM ;  ABSORPTION ;  EXCRETION ;  BACTERIUM ;  BINDING ;  RATS
语种: 英语
WOS记录号: WOS:000239868300009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139952
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Med Univ, Dalian 116027, Peoples R China

Recommended Citation:
Luan, Hongwei,Liu, Xin,Qi, Xiaohui,et al. Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail[J]. PROCESS BIOCHEMISTRY,2006,41(9):1974-1980.
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