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Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail
Luan, Hongwei; Liu, Xin; Qi, Xiaohui; Hu, Ying; Hao, Dacheng; Cui, Yu; Yang, Ling
关键词Beta-d-glucosidase Thermo- And Ph-stability Ginsenoside Rb-1 Ginsenoside Rd Purification China White Jade Snail
刊名PROCESS BIOCHEMISTRY
2006-09-01
DOI10.1016/j.procbio.2006.04.011
41期:9页:1974-1980
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]THERMOPHILIC FUNGUS ; RD ; SAPONINS ; PROTEIN ; METABOLISM ; ABSORPTION ; EXCRETION ; BACTERIUM ; BINDING ; RATS
英文摘要A highly stable beta-glucosidase from the viscera of China white jade snail was purified to apparent homogeneity. This purified glucosidase consisted of two identical subunits with a native molecular mass of approximately 230 kDa. The maximal activity to p-nitrophenyl-beta-(D)-glucopyranoside (pNPG) occurred at 70 degrees C and pH 5.6. Its most notable characteristic was stable over a wide pH range (3-11 at 30 degrees C for 24 h) and a relatively high temperature (60 degrees C for 24 h). The K-m for pNPG was 0.338 mM and the V-max was 0.25 mmol nitrophenol/min/mg glucosidase at pH 5.6 and 50 degrees C. Compared to its activity against pNPG (100%), the beta-glucosidase exhibited low levels of activity against other aryl-glycosides (less than 1%). Additionally, the enzyme hydrolyzed the 20-C, beta-(1 -> 6)-glucoside of ginsenoside Rb-1 to produce ginsenoside Rd, but did not hydrolyze the other beta-(D)-glucosidic bonds of Rb-1. The properties of the enzyme could make it become a useful tool in biotransformation of glucosides. (c) 2006 Elsevier Ltd. All rights reserved.
语种英语
WOS记录号WOS:000239868300009
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139952
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Med Univ, Dalian 116027, Peoples R China
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GB/T 7714
Luan, Hongwei,Liu, Xin,Qi, Xiaohui,et al. Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail[J]. PROCESS BIOCHEMISTRY,2006,41(9):1974-1980.
APA Luan, Hongwei.,Liu, Xin.,Qi, Xiaohui.,Hu, Ying.,Hao, Dacheng.,...&Yang, Ling.(2006).Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail.PROCESS BIOCHEMISTRY,41(9),1974-1980.
MLA Luan, Hongwei,et al."Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail".PROCESS BIOCHEMISTRY 41.9(2006):1974-1980.
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