中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Culture of neural stem cells in calcium alginate beads
作者: Li, Xiangqin;  Liu, Tianqing;  Song, Kedong;  Yao, Lisong;  Ge, Dan;  Bao, Chunyu;  Ma, Xuehu;  Cui, Zhanfeng
刊名: BIOTECHNOLOGY PROGRESS
发表日期: 2006-12-01
DOI: 10.1021/bp060185z
卷: 22, 期:6, 页:1683-1689
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
英文摘要: Neural stem cells ( NSCs) with the capacity of extensive self-renewal and multilineage differentiation have attracted more and more attention in research as NSCs will play an important role in the nerve disease treatment and nerve injury repair. The shortage of NSCs, both their sources and their numbers, however, is the biggest challenge for their clinic application, and hence, in vitro culture and expansion of NSCs is vitally important to realize their potentials. In this work, mouse-derived NSCs were cultured in three-dimensional calcium alginate beads ( Ca-Alg-Bs). Gelling conditions, cell density, and cell harvest were determined by the exploration of formation and dissociation parameters for Ca-Alg-Bs. Additionally, the recovered and the subsequent induced cells were identified by immunofluorescence staining of Nestin, beta-tubulin, and GFAP. The results show that the 2-mm diameter Ca-Alg-Bs, prepared with 1.5 % sodium alginate solution and 3.5% CaCl2 solution and with gelling for 10 min, is suitable for the NSCs culture. The seeding density of 0.8 x 10(5) cells . mL(-1) for the encapsulation of NSCs resulted in the most expansion, and the NSCs almost doubled during the experiment. The average cell recovery rate is over 88.5%, with the Ca-Alg-Bs dissolving in 55 mM sodium citrate solution for 10 min. The recovered cells cultured in the Ca-Alg-Bs still expressed Nestin and had the capacity of multilineage differentiation into neurons and glial cells and, thus, remained to be NSCs. These results demonstrate that NSC expansion within Ca-Alg-Bs is feasible and provides further possibilities for NSC expansion in bioreactors of the scale of clinical relevance.
关键词[WOS]: SPINAL-CORD ;  ANIMAL-CELLS ;  IN-VITRO ;  DIFFERENTIATION ;  BIOREACTORS ;  PROMOTION ;  ISLETS ;  MATRIX ;  DAMAGE
语种: 英语
WOS记录号: WOS:000242427200027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139961
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Res & Dev Ctr Stem Cell & Tissue Engn, Dalian 116023, Peoples R China
2.Univ Oxford, Dept Engn Sci, Oxford Ctr Tissue Engn & Bioproc, Oxford OX1 3PJ, England

Recommended Citation:
Li, Xiangqin,Liu, Tianqing,Song, Kedong,et al. Culture of neural stem cells in calcium alginate beads[J]. BIOTECHNOLOGY PROGRESS,2006,22(6):1683-1689.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Li, Xiangqin]'s Articles
 [Liu, Tianqing]'s Articles
 [Song, Kedong]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Li, Xiangqin]‘s Articles
 [Liu, Tianqing]‘s Articles
 [Song, Kedong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace