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题名: Simultaneous determination of polyphenols in tobacco by high performance liquid chromatography/photodiode array detection
作者: Bai Changmin;  Zhong Kejun;  Huang Jianguo;  Cao Guojun;  Xu Guowang
关键词: tobacco ;  polyphenol ;  ultrasonic extraction ;  high performance liquid chromatography
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2006-11-01
卷: 34, 期:11, 页:1619-1621
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Chlorogenic acid, chrysatropic acid and rutin in tobacco were analyzed using high performance liquid chromatography( HPLC) /photodiode array deteciton (PDA). After the tobacco was simply treated by ultrasonic extraction using methanol/water (4: 1, V/ V) and filtration, the obtained liquid sample was analyzed by HPLC using hypersil ODS column ( 4.6 mm x 150 min i. d., 3 mu m). Methanol/trifluoroacetic acid (TFA) aqueous solution was used as mobile phase for gradient elution. PDA spectrum was used for assistant identification. Internal standard method was applied for quantitation. The recovery was in the range of 84.4% -96.4%. The relative standard deviation of determination was 1. 4% - 6. 4% for three compounds.
关键词[WOS]: CHLOROGENIC ACID ;  RUTIN ;  SEPARATION ;  CIGARETTES ;  CAPILLARY
语种: 英语
WOS记录号: WOS:000244672100024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140029
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Changde Cigarette Factory, Ctr Sci & Technol, Changde 415000, Peoples R China
2.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Nanjing Univ Sci & Technol, Nanjing 210094, Peoples R China

Recommended Citation:
Bai Changmin,Zhong Kejun,Huang Jianguo,et al. Simultaneous determination of polyphenols in tobacco by high performance liquid chromatography/photodiode array detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(11):1619-1621.
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