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题名: Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro-sirius red staining, polarized light microscopy, and digital image analysis
作者: Zhang, H;  Sun, L;  Wang, W;  Ma, X
关键词: image analysis ;  fibrosis ;  collagen ;  microcapsules ;  polarized light microscopy ;  stains and staining
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2006
DOI: 10.1002/jbm.a.30491
卷: 76A, 期:1, 页:120-125
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Transplantation of microencapsulated cells shows potential to treat a variety of diseases. To improve the quality of microcapsules, efficient methods need to be established and standardized for the evaluation of biocompatibility of microcapsules. To this end, a microscopical method was developed to analyze quantitatively the degree of fibrotic overgrowth (FO), which serves as the index of biocompatibility. In this method, sections were stained with picro-sirius red and illuminated with a polarized light microscope. The images were digitized with the use of a computer-video system in which collagenous content was depicted by gray levels I to 255. Both the area of collagen and the thickness of FO were examined. The ratio of Type I/Type III collagen and the thickness were used as indexes of the degree of FO. The data showed that the ratio of Type I/Type III collagen and the thickness increased with time, and FO was not significant 4 weeks after transplantation. In comparison, this new method is more efficient to evaluate FO. (c) 2005 Wiley Periodicals, Inc.
关键词[WOS]: FIBROTIC OVERGROWTH ;  MICROENCAPSULATED ISLETS ;  SEMIQUANTITATIVE INDEXES ;  LIVER FIBROSIS ;  ALGINATE ;  COLLAGEN ;  BIOCOMPATIBILITY ;  IMMUNOSUPPRESSION ;  TRANSPLANTATION ;  QUANTIFICATION
语种: 英语
WOS记录号: WOS:000234105400014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140030
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, H,Sun, L,Wang, W,et al. Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro-sirius red staining, polarized light microscopy, and digital image analysis[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2006,76A(1):120-125.
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