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题名: Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer
作者: Wang, Guilan;  Yuan, Jingli;  Hai, Xiaodan;  Matsumoto, Kazuko
关键词: homogeneous immunoassay ;  3,5,3 '-triiodo-L-thyronine ;  europium ;  fluorescence resonance energy transfer
刊名: TALANTA
发表日期: 2006-08-15
DOI: 10.1016/j.talanta.2005.11.052
卷: 70, 期:1, 页:133-138
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A sensitive homogeneous time-resolved fluoroimmunoassay method for 3,5,3'-triiodo-L-thyronine (T3) based on the fluorescence resonance energy transfer (FRET) from a fluorescent Eu3+ complex, {[(4,6-dichloro-1,3,5-triazin-2-yl)amino-biphenyl-4"'-yl]-2,2':6',2"-terpyridine-6,6"-diyl}bis(methylenenitrilo) tetrakis(acetate)-Eu3+ (DTBTA-Eu3+) (lambda(ex,max)= 335 nm, lambda(em,max) = 615 nm), to an organic fluorescence dye Cy5 has been developed. The new assay system combined the use of DTBTA-Eu3+-labeled T3-bovine serum albumin (BSA) conjugate and Cy5-labeled anti-T3 monoclonal antibody for a competitive-type immunoassay. After the competitive reactions of DTBTA-Eu3+-labeled T3-BSA and T3 sample with Cy5-labeled anti-T3 antibody, the T3 concentration was measured with a time-resolved mode by monitoring the sensitized emission of Cy5 derived from FRET in a homogeneous format. The method gives the detection limit of 0.26 ng/ml. The coefficient variations of the method are less than 2.0% and the recoveries are in the range of 80-111% for serum sample measurement. The concentrations of T3 in 30 human serum samples were determined. and the results were compared with those of the independently determined by a radio-immunoassay method. A good correlation was obtained with a correlation coefficient of 0.989. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: TERBIUM ;  ASSAY ;  IMMUNOASSAYS ;  TRIIODOTHYRONINE ;  CHELATE ;  MARKERS ;  LABELS ;  TSH ;  T3
语种: 英语
WOS记录号: WOS:000239709400024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140090
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China
2.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan

Recommended Citation:
Wang, Guilan,Yuan, Jingli,Hai, Xiaodan,et al. Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer[J]. TALANTA,2006,70(1):133-138.
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