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Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer
Wang, Guilan; Yuan, Jingli; Hai, Xiaodan; Matsumoto, Kazuko
关键词Homogeneous Immunoassay Europium 3 Fluorescence Resonance Energy Transfer 5 3 '-triiodo-l-thyronine
刊名TALANTA
2006-08-15
DOI10.1016/j.talanta.2005.11.052
70期:1页:133-138
收录类别ISTP ; SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TERBIUM ; ASSAY ; IMMUNOASSAYS ; TRIIODOTHYRONINE ; CHELATE ; MARKERS ; LABELS ; TSH ; T3
英文摘要A sensitive homogeneous time-resolved fluoroimmunoassay method for 3,5,3'-triiodo-L-thyronine (T3) based on the fluorescence resonance energy transfer (FRET) from a fluorescent Eu3+ complex, {[(4,6-dichloro-1,3,5-triazin-2-yl)amino-biphenyl-4"'-yl]-2,2':6',2"-terpyridine-6,6"-diyl}bis(methylenenitrilo) tetrakis(acetate)-Eu3+ (DTBTA-Eu3+) (lambda(ex,max)= 335 nm, lambda(em,max) = 615 nm), to an organic fluorescence dye Cy5 has been developed. The new assay system combined the use of DTBTA-Eu3+-labeled T3-bovine serum albumin (BSA) conjugate and Cy5-labeled anti-T3 monoclonal antibody for a competitive-type immunoassay. After the competitive reactions of DTBTA-Eu3+-labeled T3-BSA and T3 sample with Cy5-labeled anti-T3 antibody, the T3 concentration was measured with a time-resolved mode by monitoring the sensitized emission of Cy5 derived from FRET in a homogeneous format. The method gives the detection limit of 0.26 ng/ml. The coefficient variations of the method are less than 2.0% and the recoveries are in the range of 80-111% for serum sample measurement. The concentrations of T3 in 30 human serum samples were determined. and the results were compared with those of the independently determined by a radio-immunoassay method. A good correlation was obtained with a correlation coefficient of 0.989. (c) 2005 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000239709400024
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140090
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China
2.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
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Wang, Guilan,Yuan, Jingli,Hai, Xiaodan,et al. Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer[J]. TALANTA,2006,70(1):133-138.
APA Wang, Guilan,Yuan, Jingli,Hai, Xiaodan,&Matsumoto, Kazuko.(2006).Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer.TALANTA,70(1),133-138.
MLA Wang, Guilan,et al."Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer".TALANTA 70.1(2006):133-138.
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