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题名: Mass transfer kinetics and breakthrough and elution curves for bovine serum albumin using cibacron blue cellulose membranes
作者: Hao, WQ;  Wang, JD;  Zhang, XM
关键词: membrane affinity chromatography ;  mass transfer ;  pore model ;  lumped kinetic model
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2006-05-05
DOI: 10.1016/j.chroma.2006.02.047
卷: 1114, 期:1, 页:123-131
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The mass transfer of bovine serum albumin onto a stack of cibacron blue cellulose membranes in the loading and elution stages were studied. The breakthrough curves obtained in the loading stage were fitted to the pore and the lumped kinetic (LK) models, respectively. Then experimental data obtained in the elution stage were described by using the LK model in which the kinetic equation, the initial and the boundary conditions were rewritten according to the operation. For the breakthrough curves it was found that the contribution of the sorption kinetics to band broadening was significant whereas that of axial dispersion was negligible. In contrast, both of these contributions were significant to the profile of the elution curve. By studying the mass transfer kinetics in the elution stage, information about the influence of the module geometry on the performance of affinity membrane separations may be obtained. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: TRANSFER RATE COEFFICIENT ;  AFFINITY-CHROMATOGRAPHY ;  FRONTAL ANALYSIS ;  CONCENTRATION-DEPENDENCE ;  NONLINEAR CHROMATOGRAPHY ;  LIQUID-CHROMATOGRAPHY ;  MATHEMATICAL-ANALYSIS ;  MULTI-PLATE ;  PURIFICATION ;  PERFORMANCE
语种: 英语
WOS记录号: WOS:000236948800015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140091
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

Recommended Citation:
Hao, WQ,Wang, JD,Zhang, XM. Mass transfer kinetics and breakthrough and elution curves for bovine serum albumin using cibacron blue cellulose membranes[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1114(1):123-131.
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