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题名: Shape-specific detection based on fluorescence resonance energy transfer using a flexible water-soluble conjugated polymer
作者: Lv, Wei;  Li, Na;  Li, Yuliang;  Li, Ye;  Xia, Andong
刊名: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
发表日期: 2006-08-09
DOI: 10.1021/ja063004y
卷: 128, 期:31, 页:10281-10287
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: We present the detection of the shape-specific conformation of DNA based on the fluorescence resonance energy transfer (FRET) by using a novel flexible water-soluble cationic conjugated polymer (CCP). The flexible backbone of CCP has more conformational freedom with the potential to be responsive to analyte shape by electrostatic interaction between flexible CCP and negatively charged DNA. The analyte shape dependent recognition is accomplished by structural changes that compressed or extended the flexible CCP. The morphology-dependent spectral properties of the novel flexible polymer related to the analyte shapes are investigated in detail, where two types of chromophores, referred to as "isolated" segment and "packed" segment aggregates, within the flexible polymer are identified by means of ensemble and single molecule measurements upon binding with different geometric DNA. The change in fluorescence intensity upon binding with shape-specific DNA without obvious color shifts makes this novel flexible polymer a suitable CCP donor for FRET measurements. The results provide insights for understanding the spectral properties of flexible water-soluble CCP and CCP/DNA interaction related to the geometry of target analyte.
关键词[WOS]: NUCLEIC-ACID PROBES ;  CONFORMATIONAL FLUCTUATIONS ;  POLY(PHENYLENE VINYLENE) ;  SIGNAL AMPLIFICATION ;  OPTICAL-PROPERTIES ;  CHEMICAL SENSORS ;  DNA DETECTION ;  COMPLEXES ;  SPECTROSCOPY ;  BIOSENSORS
语种: 英语
WOS记录号: WOS:000239454800054
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140120
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100080, Peoples R China

Recommended Citation:
Lv, Wei,Li, Na,Li, Yuliang,et al. Shape-specific detection based on fluorescence resonance energy transfer using a flexible water-soluble conjugated polymer[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2006,128(31):10281-10287.
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