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Shape-specific detection based on fluorescence resonance energy transfer using a flexible water-soluble conjugated polymer
Lv, Wei; Li, Na; Li, Yuliang; Li, Ye; Xia, Andong
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2006-08-09
DOI10.1021/ja063004y
128期:31页:10281-10287
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]NUCLEIC-ACID PROBES ; CONFORMATIONAL FLUCTUATIONS ; POLY(PHENYLENE VINYLENE) ; SIGNAL AMPLIFICATION ; OPTICAL-PROPERTIES ; CHEMICAL SENSORS ; DNA DETECTION ; COMPLEXES ; SPECTROSCOPY ; BIOSENSORS
英文摘要We present the detection of the shape-specific conformation of DNA based on the fluorescence resonance energy transfer (FRET) by using a novel flexible water-soluble cationic conjugated polymer (CCP). The flexible backbone of CCP has more conformational freedom with the potential to be responsive to analyte shape by electrostatic interaction between flexible CCP and negatively charged DNA. The analyte shape dependent recognition is accomplished by structural changes that compressed or extended the flexible CCP. The morphology-dependent spectral properties of the novel flexible polymer related to the analyte shapes are investigated in detail, where two types of chromophores, referred to as "isolated" segment and "packed" segment aggregates, within the flexible polymer are identified by means of ensemble and single molecule measurements upon binding with different geometric DNA. The change in fluorescence intensity upon binding with shape-specific DNA without obvious color shifts makes this novel flexible polymer a suitable CCP donor for FRET measurements. The results provide insights for understanding the spectral properties of flexible water-soluble CCP and CCP/DNA interaction related to the geometry of target analyte.
语种英语
WOS记录号WOS:000239454800054
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被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140120
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100080, Peoples R China
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Lv, Wei,Li, Na,Li, Yuliang,et al. Shape-specific detection based on fluorescence resonance energy transfer using a flexible water-soluble conjugated polymer[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2006,128(31):10281-10287.
APA Lv, Wei,Li, Na,Li, Yuliang,Li, Ye,&Xia, Andong.(2006).Shape-specific detection based on fluorescence resonance energy transfer using a flexible water-soluble conjugated polymer.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,128(31),10281-10287.
MLA Lv, Wei,et al."Shape-specific detection based on fluorescence resonance energy transfer using a flexible water-soluble conjugated polymer".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 128.31(2006):10281-10287.
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