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题名: Analysis of organic acids in hawthorn by reversed phase high performance liquid chromatography
作者: Jin Gaowa;  Zhang Feifang;  Xue Xingya;  Xu Qing;  Liang Xinmiao
关键词: high performance liquid chromatography ;  hawthorn ;  organic acids
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2006-07-01
卷: 34, 期:7, 页:987-990
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Hawthorn is a traditional Chinese medicine and fruit. It contains numerous organic acids. It is reported that organic acids have the ability to cure indigestion and have many other functions. A quantitative reversed-phase high performance liquid chromatography method using diode array detector has been developed for the determination of citric acid and malic acid in hawthorn. A Hypersil ODS2 column was used with phosphate buffer solution ( pH = 2. 6) as the mobile phase at a flow rate of 0.8 mL/min. The column temperature was 25 degrees C and the UV detection wavelength was 210 nm. The calibration curve for citric acid and malic acid was in good linearity in the range of 0.50 - 50.04 mu g with the regression equation Y = 78. 2X - 0.08, r = 0.9999 and 0.10 - 10.00 mu g, Y = 65.1X - 0.065, r = 0.9999, respectively. The average recoveries of three concentration leveds for citric acid and malic acid in hawthorn were 95.5% - 96.6% and 100.2% 103.7%, respectively. RSD were 0.11% - 0.38% and 0.41% - 53%, respectively. The detection limits for citric acid and malic acid were 0.04 mu g and 0.03 mu g (S/N 3), respectively. The results indicate that this method is fast, sensitive, reproducible and practical for the routine analysis of organic acids in hawthorn.
语种: 英语
WOS记录号: WOS:000242858300021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140131
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Jin Gaowa,Zhang Feifang,Xue Xingya,et al. Analysis of organic acids in hawthorn by reversed phase high performance liquid chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(7):987-990.
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