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题名: A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis
作者: Mao, Xiuli;  Chu, Ivan K.;  Lin, Bingcheng
关键词: glycopeptides ;  glycoprotein ;  microchip electrophoresis ;  MS ;  nanoelectrospray interface
刊名: ELECTROPHORESIS
发表日期: 2006-12-01
DOI: 10.1002/elps.200600349
卷: 27, 期:24, 页:5059-5067
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Microchip was coupled with MS through a stable, sensitive, and controllable sheath-flow nanoelectrospray (nES) interface for glycoprotein and glycopeptide analysis. The nano-ESI (nESI) was made with a delivery capillary, a commercial nES capillary, and a stainless steel (SS) tube which were connected together through a tee unit. High voltage for nES was applied on the SS tube and the commercial nES capillary was used as nES emitter. The delivery capillary was attached to the microchannel for delivering liquid from microchip to the nESI source. The flow rate of sheath liquid was optimized to be 100-200 nL/min which largely reduced the sample dilution. The detection limit of peptides on this microchip/MS platform was at femtomole level. Glycoprotein and glycopeptides were also successfully analyzed on the platform. All the glycoforms and glycopeptides of ribonuclease B (RNase B) were identified with this method. Some structures of the glycopeptides from RNase B were further characterized with MS/MS on the microchip, coupled with a quadrupole IT-MS.
关键词[WOS]: ELECTROSPRAY MASS-SPECTROMETRY ;  ON-A-CHIP ;  MICROFLUIDIC DEVICES ;  MALDI-MS ;  CAPILLARY ;  SEPARATION ;  PERFORMANCE ;  EMITTERS ;  SYSTEM
语种: 英语
WOS记录号: WOS:000243304100022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140134
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
3.Chinese Acad Sci, Grad Sch, Dalian, Peoples R China
4.Univ Hong Kong, Genome Res Ctr, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Mao, Xiuli,Chu, Ivan K.,Lin, Bingcheng. A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis[J]. ELECTROPHORESIS,2006,27(24):5059-5067.
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