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题名: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
作者: Yu Fu;  Xu, Songyun;  Pan, Chensong;  Ye, Mingliang;  Zou, Hanfa;  Guo, Baochuan
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2006
DOI: 10.1093/nar/gkl509
卷: 34, 期:13
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: A new matrix of 3,4-diaminobenzophenone (DABP) was demonstrated to be advantageous in the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. With DABP as a matrix, intact oligonucleotide ions can be readily produced with lower laser powers, resulting in better detection limits, less fragmentation and fewer alkali metal ion adducts compared with the results obtained with conventional matrices. Importantly, minimal fragmentation and fewer alkali metal ion adducts were seen even at low concentrations of oligonucleotides. It was also found that samples prepared with DABP are highly homogenous and therefore reducing the need for finding 'sweet' spots in MALDI. In addition, excellent shot-to-shot reproducibility, resolution and signal-to-noise ratio were seen with DABP as the matrix.
关键词[WOS]: UV-MALDI MS ;  CPG METHYLATION ;  NUCLEIC-ACIDS ;  ION STABILITY ;  STRANDED-DNA ;  CO-MATRICES ;  DESORPTION ;  TOF ;  OLIGODEOXYNUCLEOTIDES ;  ADDITIVES
语种: 英语
WOS记录号: WOS:000240583100005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140135
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China
2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA

Recommended Citation:
Yu Fu,Xu, Songyun,Pan, Chensong,et al. A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J]. NUCLEIC ACIDS RESEARCH,2006,34(13).
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