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Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis
Ran, CQ; Yu, XJ; Jin, MF; Zhang, W
Source PublicationBIOTECHNOLOGY PROGRESS
2006-03-01
DOI10.1021/bp050289u
Volume22Issue:2Pages:438-443
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS KeywordPHOTOSYNTHETIC ELECTRON-TRANSPORT ; WATERSPLITTING ENZYME-SYSTEM ; CHLAMYDOMONAS-REINHARDTII ; DEACTIVATION REACTIONS ; EVOLUTION ; PHOTOPRODUCTION ; SCENEDESMUS ; H-2
AbstractWe demonstrated that a significant volume of H-2 gas could be photobiologically produced by a marine green alga Platymonas subcordiformis when an uncoupler of photophosphorylation, carbonyl cyanide in-chlorophenylhydrazone (CCCP), was added after 32 h of anaerobic dark incubation, whereas a negligible volume of H-2 gas was produced without CCCP. The role of CCCP in enhancing photobiological H-2 production was delineated. CCCP as an ADRY agent (agent accelerating the deactivation reactions of water-splitting enzyme system Y) rapidly inhibited the photosystem H (PSII) activity of P. subcordiformis cells, resulting in a markedly decline in the coupled oxygen evolution. The mitochondrial oxidative respiration was only slightly inactivated by CCCP, which depleted O-2 in the light. As a result, anaerobiosis during the stage of photobiological H-2 evolution was established, preventing severe O-2 inactivation of the reversible hydrogenase in P. subcordiformis. The uncoupling effect of CCCP accelerates electron transfer from water due to a disruption of the proton motive force and release of Delta pH across the thylakoid membrane and thus enhances the accessibility of electron and H+ to hydrogenase. The electrons for hydrogen photoevolution are mainly from the photolysis of water (90%). Upon the addition of CCCP, Chl a/b ratio increased, which implies a decrease in the light-harvesting PSII antennae or an increase in PSII/PSI ratio, possibly resulting in higher efficiency of utilization of light energy. The enhancement of H-2 evolution by the addition of CCCP is mostly due to the combination of the above three mechanisms. However, the disruption of the proton gradient across the thylakoid membrane may prevent a sustained photobiological H-2 evolution due to a shortfall of ATP generation essential for the maintenance and repair functions of the cells.
Language英语
WOS IDWOS:000236783400015
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140142
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
Recommended Citation
GB/T 7714
Ran, CQ,Yu, XJ,Jin, MF,et al. Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis[J]. BIOTECHNOLOGY PROGRESS,2006,22(2):438-443.
APA Ran, CQ,Yu, XJ,Jin, MF,&Zhang, W.(2006).Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis.BIOTECHNOLOGY PROGRESS,22(2),438-443.
MLA Ran, CQ,et al."Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis".BIOTECHNOLOGY PROGRESS 22.2(2006):438-443.
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