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题名: Fast extraction, amplification and analysis of genes from human blood
作者: Zhang, LH;  Dang, FQ;  Kaji, N;  Baba, Y
关键词: gene analysis ;  polymerase chain reaction ;  microchip electrophoresis
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2006-02-17
DOI: 10.1016/j.chroma.2005.10.071
卷: 1106, 期:1-2, 页:175-180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast DNA extraction and purification by Generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. With this method, 3 mu L blood was enough to obtain adequate target fragments in human genes. Under the optimal conditions in each step, the sample preparation for eight fragments in P-globin gene and four fragments in ras gene could be finished within 20 min. Since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. In addition, other advantages such as fast speed and low consumption of samples were demonstrated. All these merits proved that such a combination method was of great potential for the clinical diagnostics. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: POLYMERASE-CHAIN-REACTION ;  CAPILLARY-ELECTROPHORESIS ;  SAMPLE PREPARATION ;  REACTION PRODUCTS ;  MICROCHIP ELECTROPHORESIS ;  PCR PRODUCTS ;  DNA ANALYSIS ;  GENOMIC DNA ;  POLYMORPHISMS ;  POPULATION
语种: 英语
WOS记录号: WOS:000235256800029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140162
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Tokushima, Grad Sch Pharmaceut Sci, CREST, JST,Dept Mol & Pharmaceut Biotechnol, Tokushima 7708505, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116023, Peoples R China
3.Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Chikusa Ku, Nagoya, Aichi 4648603, Japan
4.Furuno Elect Co Ltd, Nishinomiya, Hyogo 6628580, Japan
5.AIST, Hlth Technol Res Ctr, Takamatsu, Kagawa 7610395, Japan

Recommended Citation:
Zhang, LH,Dang, FQ,Kaji, N,et al. Fast extraction, amplification and analysis of genes from human blood[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1106(1-2):175-180.
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