DICP OpenIR
Fast extraction, amplification and analysis of genes from human blood
Zhang, LH; Dang, FQ; Kaji, N; Baba, Y
关键词Gene Analysis Polymerase Chain Reaction Microchip Electrophoresis
刊名JOURNAL OF CHROMATOGRAPHY A
2006-02-17
DOI10.1016/j.chroma.2005.10.071
1106期:1-2页:175-180
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]POLYMERASE-CHAIN-REACTION ; CAPILLARY-ELECTROPHORESIS ; SAMPLE PREPARATION ; REACTION PRODUCTS ; MICROCHIP ELECTROPHORESIS ; PCR PRODUCTS ; DNA ANALYSIS ; GENOMIC DNA ; POLYMORPHISMS ; POPULATION
英文摘要In order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast DNA extraction and purification by Generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. With this method, 3 mu L blood was enough to obtain adequate target fragments in human genes. Under the optimal conditions in each step, the sample preparation for eight fragments in P-globin gene and four fragments in ras gene could be finished within 20 min. Since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. In addition, other advantages such as fast speed and low consumption of samples were demonstrated. All these merits proved that such a combination method was of great potential for the clinical diagnostics. (c) 2005 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000235256800029
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140162
专题中国科学院大连化学物理研究所
作者单位1.Univ Tokushima, Grad Sch Pharmaceut Sci, CREST, JST,Dept Mol & Pharmaceut Biotechnol, Tokushima 7708505, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116023, Peoples R China
3.Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Chikusa Ku, Nagoya, Aichi 4648603, Japan
4.Furuno Elect Co Ltd, Nishinomiya, Hyogo 6628580, Japan
5.AIST, Hlth Technol Res Ctr, Takamatsu, Kagawa 7610395, Japan
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Zhang, LH,Dang, FQ,Kaji, N,et al. Fast extraction, amplification and analysis of genes from human blood[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1106(1-2):175-180.
APA Zhang, LH,Dang, FQ,Kaji, N,&Baba, Y.(2006).Fast extraction, amplification and analysis of genes from human blood.JOURNAL OF CHROMATOGRAPHY A,1106(1-2),175-180.
MLA Zhang, LH,et al."Fast extraction, amplification and analysis of genes from human blood".JOURNAL OF CHROMATOGRAPHY A 1106.1-2(2006):175-180.
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