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Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis
Zhu, G.; Zhang, L.; Liang, Z.; Zhang, W.; Zhang, Y.
关键词Capillary Isoelectric Focusing Monolithic Immobilized Ph Gradient Polyacrylamide Protein
刊名MOLECULAR & CELLULAR PROTEOMICS
2006-10-01
DOI10.1002/elps.200600189
5期:10页:S141-S141
收录类别SCI
文章类型Meeting Abstract
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]PERFORMANCE
英文摘要Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate, and N,N'-methylenebisacrylamide in the presence of 1,4-butanediol, dodecanol, and DMSO as porogens. With Ampholine attached to the surface of the porous monolith via epoxide groups, a monolithic-IPG (M-IPG) was formed and showed good mechanical and chemical stability. With such a column immobilized by Ampholine 3.5-10, IEF-MIX 3.6-9.3 was separated and good linearity was obtained. The CIEF behavior of M-IPG was evinced by comparing the current with that in the open tubular capillary. In addition, the protein mixtures excreted from lung cancer cells of rats were analyzed with such a new M-IPG column.
语种英语
WOS记录号WOS:000241506400323
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140192
专题中国科学院大连化学物理研究所
作者单位CAS, Dalian Inst Chem Phys, Dalian, Peoples R China
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GB/T 7714
Zhu, G.,Zhang, L.,Liang, Z.,et al. Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis[J]. MOLECULAR & CELLULAR PROTEOMICS,2006,5(10):S141-S141.
APA Zhu, G.,Zhang, L.,Liang, Z.,Zhang, W.,&Zhang, Y..(2006).Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis.MOLECULAR & CELLULAR PROTEOMICS,5(10),S141-S141.
MLA Zhu, G.,et al."Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis".MOLECULAR & CELLULAR PROTEOMICS 5.10(2006):S141-S141.
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