中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis
作者: Zhu, G.;  Zhang, L.;  Liang, Z.;  Zhang, W.;  Zhang, Y.
关键词: capillary isoelectric focusing ;  monolithic immobilized pH gradient ;  polyacrylamide ;  protein
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2006-10-01
DOI: 10.1002/elps.200600189
卷: 5, 期:10, 页:S141-S141
收录类别: SCI
文章类型: Meeting Abstract
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate, and N,N'-methylenebisacrylamide in the presence of 1,4-butanediol, dodecanol, and DMSO as porogens. With Ampholine attached to the surface of the porous monolith via epoxide groups, a monolithic-IPG (M-IPG) was formed and showed good mechanical and chemical stability. With such a column immobilized by Ampholine 3.5-10, IEF-MIX 3.6-9.3 was separated and good linearity was obtained. The CIEF behavior of M-IPG was evinced by comparing the current with that in the open tubular capillary. In addition, the protein mixtures excreted from lung cancer cells of rats were analyzed with such a new M-IPG column.
关键词[WOS]: PERFORMANCE
语种: 英语
WOS记录号: WOS:000241506400323
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140192
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.CAS, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Zhu, G.,Zhang, L.,Liang, Z.,et al. Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis[J]. MOLECULAR & CELLULAR PROTEOMICS,2006,5(10):S141-S141.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhu, G.]'s Articles
 [Zhang, L.]'s Articles
 [Liang, Z.]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhu, G.]‘s Articles
 [Zhang, L.]‘s Articles
 [Liang, Z.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace