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题名: Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography
作者: Qu, Qishu;  Lu, Xiao;  Huang, Xiaojing;  Hu, Xiaoya;  Zhang, Yukui;  Yan, Chao
关键词: capillary column ;  pressurized capillary electrochromatography ;  silica
刊名: ELECTROPHORESIS
发表日期: 2006-10-01
DOI: 10.1002/elps.20060012
卷: 27, 期:20, 页:3981-3987
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Nonporous silica spheres (1 mu m) were synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 gm id for a length of 20 cm, which was evaluated by using pressurized CEC (pCEC). The efficiency of the C18 RP column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. The effects of experimental parameters such as the applied voltage, sample size, pump flow rate, pH value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. Column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/L phosphate buffer (pH 7.8) at an applied voltage of 10 kV and a supplementary pressure of 500 psi.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  COLUMN TECHNOLOGY ;  SEPARATION ;  EFFICIENCY ;  ELECTROPHORESIS ;  PEPTIDES ;  PACKING
语种: 英语
WOS记录号: WOS:000241916100008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140204
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Yangzhou Univ, Jiangsu Key Lab Environm Mat & Environm Engn, Coll Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China
2.Unimicro Technol Inc, Pleasanton, CA USA
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China

Recommended Citation:
Qu, Qishu,Lu, Xiao,Huang, Xiaojing,et al. Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography[J]. ELECTROPHORESIS,2006,27(20):3981-3987.
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