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Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography
Qu, Qishu; Lu, Xiao; Huang, Xiaojing; Hu, Xiaoya; Zhang, Yukui; Yan, Chao
KeywordCapillary Column Pressurized Capillary Electrochromatography Silica
Source PublicationELECTROPHORESIS
2006-10-01
DOI10.1002/elps.20060012
Volume27Issue:20Pages:3981-3987
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; COLUMN TECHNOLOGY ; SEPARATION ; EFFICIENCY ; ELECTROPHORESIS ; PEPTIDES ; PACKING
AbstractNonporous silica spheres (1 mu m) were synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 gm id for a length of 20 cm, which was evaluated by using pressurized CEC (pCEC). The efficiency of the C18 RP column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. The effects of experimental parameters such as the applied voltage, sample size, pump flow rate, pH value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. Column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/L phosphate buffer (pH 7.8) at an applied voltage of 10 kV and a supplementary pressure of 500 psi.
Language英语
WOS IDWOS:000241916100008
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140204
Collection中国科学院大连化学物理研究所
Affiliation1.Yangzhou Univ, Jiangsu Key Lab Environm Mat & Environm Engn, Coll Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China
2.Unimicro Technol Inc, Pleasanton, CA USA
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Qu, Qishu,Lu, Xiao,Huang, Xiaojing,et al. Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography[J]. ELECTROPHORESIS,2006,27(20):3981-3987.
APA Qu, Qishu,Lu, Xiao,Huang, Xiaojing,Hu, Xiaoya,Zhang, Yukui,&Yan, Chao.(2006).Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography.ELECTROPHORESIS,27(20),3981-3987.
MLA Qu, Qishu,et al."Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography".ELECTROPHORESIS 27.20(2006):3981-3987.
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