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Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography
Qu, Qishu; Lu, Xiao; Huang, Xiaojing; Hu, Xiaoya; Zhang, Yukui; Yan, Chao
关键词Capillary Column Pressurized Capillary Electrochromatography Silica
刊名ELECTROPHORESIS
2006-10-01
DOI10.1002/elps.20060012
27期:20页:3981-3987
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; COLUMN TECHNOLOGY ; SEPARATION ; EFFICIENCY ; ELECTROPHORESIS ; PEPTIDES ; PACKING
英文摘要Nonporous silica spheres (1 mu m) were synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 gm id for a length of 20 cm, which was evaluated by using pressurized CEC (pCEC). The efficiency of the C18 RP column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. The effects of experimental parameters such as the applied voltage, sample size, pump flow rate, pH value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. Column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/L phosphate buffer (pH 7.8) at an applied voltage of 10 kV and a supplementary pressure of 500 psi.
语种英语
WOS记录号WOS:000241916100008
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140204
专题中国科学院大连化学物理研究所
作者单位1.Yangzhou Univ, Jiangsu Key Lab Environm Mat & Environm Engn, Coll Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China
2.Unimicro Technol Inc, Pleasanton, CA USA
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China
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GB/T 7714
Qu, Qishu,Lu, Xiao,Huang, Xiaojing,et al. Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography[J]. ELECTROPHORESIS,2006,27(20):3981-3987.
APA Qu, Qishu,Lu, Xiao,Huang, Xiaojing,Hu, Xiaoya,Zhang, Yukui,&Yan, Chao.(2006).Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography.ELECTROPHORESIS,27(20),3981-3987.
MLA Qu, Qishu,et al."Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography".ELECTROPHORESIS 27.20(2006):3981-3987.
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